Log In Sign up
The largest database of trusted experimental protocols

Eclipse ti

Manufactured by PerkinElmer
Visit Supplier

The Eclipse Ti is a high-performance fluorescence microscope system from PerkinElmer. It is designed for advanced imaging applications in life science research. The Eclipse Ti features a modular design, allowing for customization to meet specific experimental requirements.

Automatically generated - may contain errors

Lab products found in correlation

Ultraview vox confocal system, by PerkinElmer (4 mentions) Em ccd camera, by Hamamatsu Photonics (4 mentions) Na apo tirf objective, by Nikon (2 mentions) Na plan apo objective, by Nikon (2 mentions) P35g 1.5 20 c, by MatTek (2 mentions) Na apochromat tirf objective, by Nikon (2 mentions) Na plan apochromat oil immersion objective, by Nikon (2 mentions) Professional v 19, by Scientific Volume Imaging (2 mentions) Nis elements ar v4, by Nikon (2 mentions) Ultra 897, by Oxford Instruments (2 mentions) Volocity acquisition software, by PerkinElmer (2 mentions)

Spelling variants of this product

Eclipse Ti-E (4 citations) ECLIPSE Ti microscope (2 citations)

4 protocols using eclipse ti

1

Live-Cell Confocal Imaging Techniques

Check if the same lab product or an alternative is used in the 5 most similar protocols
SDC microscopy was performed utilizing a Nikon Eclipse Ti inverted microscope equipped with an UltraVIEW VOX confocal system (Perkin Elmer) and an EM-CCD camera (Hamamatsu) housed within a sealed chamber at 37°C. Cells in their native medium were excited using laser wavelengths of 405 nm, 488 nm, 561 nm, and/or 640 nm and fluorescence was detected by either a 100X/1.49NA apo TIRF objective (Nikon) or 60X/1.4NA Plan Apo objective (Nikon) with standard emission filters. For confocal imaging of live submerged cultures, cells were transduced and/or transfected with fluorophore-tagged constructs and then seeded into glass-bottom dishes (MatTek #P35G-1.5–20-C) at least 24 hr prior to imaging in their native medium. For imaging of live stratified cultures, organotypic epidermis was transferred from the transwell into a 35 mm glass-bottom dish with the top of the tissue against the glass. Multi-color Z stack images were acquired using a piezo motor with a step size of 200 nm.
Simpson C.L., Tokito M.K., Uppala R., Sarkar M.K., Gudjonsson J.E, & Holzbaur E.L. (2021). NIX initiates mitochondrial fragmentation via DRP1 to drive epidermal differentiation. Cell reports, 34(5), 108689.
+ Open protocol
+ Expand
2

Live-Cell Confocal Imaging Techniques

Check if the same lab product or an alternative is used in the 5 most similar protocols
SDC microscopy was performed utilizing a Nikon Eclipse Ti inverted microscope equipped with an UltraVIEW VOX confocal system (Perkin Elmer) and an EM-CCD camera (Hamamatsu) housed within a sealed chamber at 37°C. Cells in their native medium were excited using laser wavelengths of 405 nm, 488 nm, 561 nm, and/or 640 nm and fluorescence was detected by either a 100X/1.49NA apo TIRF objective (Nikon) or 60X/1.4NA Plan Apo objective (Nikon) with standard emission filters. For confocal imaging of live submerged cultures, cells were transduced and/or transfected with fluorophore-tagged constructs and then seeded into glass-bottom dishes (MatTek #P35G-1.5–20-C) at least 24 hr prior to imaging in their native medium. For imaging of live stratified cultures, organotypic epidermis was transferred from the transwell into a 35 mm glass-bottom dish with the top of the tissue against the glass. Multi-color Z stack images were acquired using a piezo motor with a step size of 200 nm.
Simpson C.L., Tokito M.K., Uppala R., Sarkar M.K., Gudjonsson J.E, & Holzbaur E.L. (2021). NIX initiates mitochondrial fragmentation via DRP1 to drive epidermal differentiation. Cell reports, 34(5), 108689.
+ Open protocol
+ Expand
3

Spinning-Disk Confocal Microscopy Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spinning-disk confocal microscopy (SDCM) was performed on one of two systems. SDCM #1 was a Nikon Eclipse Ti inverted scope equipped with PerkinElmer UltraVIEW VOX confocal system (Perkin Elmer) and an EM-CCD camera (Hamamatsu) housed within a 37°C environment chamber. Cells were excited with 405nm, 488nm, 561nm, and 640nm laser lines, and emission was collected using either a 100X/1.49NA apochromat TIRF objective (Nikon) or 60X/1.4NA plan apochromat oil immersion objective (Nikon) with standard emission filter sets. Images were acquired with Volocity acquisition software (Perkin Elmer). Z-stacks were acquired using a piezo motor and step sizes of either 100 nm or 150 nm, requiring ~200–300 steps to fully sample rounded, metaphase cells. 3D image stacks were deconvolved with Huygens Professional v. 19.04 (Scientific Volume Imaging) with the CMLE algorithm and signal-to-noise ratio between 20 and 30, maximum of 40 iterations, and stopping criterion of 0.01. SDCM #2 was a NikonTiE inverted microscope equipped with a spinning-disk scan head (Yokogawa, CSU-X1) and an EM-CCD camera (iXon Ultra 897). Imaging was performed with standard laser-lines with fluorescence emission collected by a 100X/1.49NA apochromat TIRF objective (Nikon). Images were acquired with NIS-Elements AR v4.40.00 (Nikon). Cells were incubated within a Tokai Hit stage top incubator at 37 °C and 5% CO2.
Moore A.S., Coscia S.M., Simpson C.L., Ortega F.E., Wait E.C., Heddleston J.M., Nirschl J.J., Obara C.J., Guedes-Dias P., Boecker C.A., Chew T.L., Theriot J.A., Lippincott-Schwartz J, & Holzbaur E.L. (2021). Actin cables and comet tails organize mitochondrial networks in mitosis. Nature, 591(7851), 659-664.
+ Open protocol
+ Expand
4

Spinning-Disk Confocal Microscopy Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spinning-disk confocal microscopy (SDCM) was performed on one of two systems. SDCM #1 was a Nikon Eclipse Ti inverted scope equipped with PerkinElmer UltraVIEW VOX confocal system (Perkin Elmer) and an EM-CCD camera (Hamamatsu) housed within a 37°C environment chamber. Cells were excited with 405nm, 488nm, 561nm, and 640nm laser lines, and emission was collected using either a 100X/1.49NA apochromat TIRF objective (Nikon) or 60X/1.4NA plan apochromat oil immersion objective (Nikon) with standard emission filter sets. Images were acquired with Volocity acquisition software (Perkin Elmer). Z-stacks were acquired using a piezo motor and step sizes of either 100 nm or 150 nm, requiring ~200–300 steps to fully sample rounded, metaphase cells. 3D image stacks were deconvolved with Huygens Professional v. 19.04 (Scientific Volume Imaging) with the CMLE algorithm and signal-to-noise ratio between 20 and 30, maximum of 40 iterations, and stopping criterion of 0.01. SDCM #2 was a NikonTiE inverted microscope equipped with a spinning-disk scan head (Yokogawa, CSU-X1) and an EM-CCD camera (iXon Ultra 897). Imaging was performed with standard laser-lines with fluorescence emission collected by a 100X/1.49NA apochromat TIRF objective (Nikon). Images were acquired with NIS-Elements AR v4.40.00 (Nikon). Cells were incubated within a Tokai Hit stage top incubator at 37 °C and 5% CO2.
Moore A.S., Coscia S.M., Simpson C.L., Ortega F.E., Wait E.C., Heddleston J.M., Nirschl J.J., Obara C.J., Guedes-Dias P., Boecker C.A., Chew T.L., Theriot J.A., Lippincott-Schwartz J, & Holzbaur E.L. (2021). Actin cables and comet tails organize mitochondrial networks in mitosis. Nature, 591(7851), 659-664.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!