The starting feedstock comprised mixtures of as-supplied iron(II,III) oxide powder (Fe3O4; ≤5 µm, 95%; Merck, Gillingham, UK) and calcium carbonate as porogen (CaCO3, ≤5 µm, 98%; Fisher Scientific UK Ltd, Loughborough, UK.). The magnetite (Fe3O4) powder was mixed with CaCO3 using a pestle and mortar and combined with droplets of 2% aqueous solution polyvinyl alcohol (PVA; Merck, UK) to act as a binder, followed by drying at 37 °C for 24 h.
Fe3o4
Manufactured by Merck Group
Sourced in United States, United Kingdom, Germany
Fe3O4 is a type of iron oxide compound. It is a dark, magnetic material that is commonly used in various scientific and industrial applications. Fe3O4 exhibits unique physical and chemical properties that make it suitable for a range of laboratory equipment and instrumentation.
Automatically generated - may contain errors
Lab products found in correlation
Caco3, by Merck Group (2 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Fecl2 4h2o, by Merck Group (1 mentions)
Nh4oh, by Merck Group (1 mentions)
Mptms, by Merck Group (1 mentions)
Fecl3 6h2o, by Merck Group (1 mentions)
Tetraethyl orthosilicate, by Merck Group (1 mentions)
Ecoflex 00 30 part a, by Smooth-On (1 mentions)
Ecoflex 0030 part b, by Smooth-On (1 mentions)
Graphene nanoplatelet, by Merck Group (1 mentions)
Al2o3, by Merck Group (1 mentions)
Aspirin asa, by Merck Group (1 mentions)
Nah2po4, by Merck Group (1 mentions)
Na2hpo4, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Zn3 po4 2, by Merck Group (1 mentions)
Low melting point agarose type 7, by Merck Group (1 mentions)
Smooth muscle basal medium 2, by PromoCell (1 mentions)
Fetal calf serum (fcs), by Merck Group (1 mentions)
Collagenase, by Merck Group (1 mentions)
23 protocols using fe3o4
1
Magnetite-Calcium Carbonate Composite Synthesis
2
Synthesis and Coating of Magnetite
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Chemicals used included FeCl2.4H2O, FeCl3.6H2O, HCl 37%, and NH4OH 25% supplied from Merck as receipted without any prior treatment for preparation of magnetite (Fe3O4), and commercial Fe3O4 from Aldrich used for a control material. For coating the magnetite was used Na2SiO3 solution (13% SiO2) produced from treatment of rice hull ash and mercaptopropyltrimethoxysilane (MPTMS) from Merck.
3
Fabrication of Soft Magnetic Composite Fibers
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The neodymium–iron–boron (NdFeB) nanomagnets (Magnequench) were firstly coated with a layer of SiO2 by hydrolysis and polycondensation of tetraethyl orthosilicate (Sigma Aldrich). Subsequently, NdFeB nanomagnets were mixed with Ecoflex 00-30 part A (Smooth-on Inc.). Then, the Ecoflex 00-30 part B (Smooth-on Inc.) was added to the mixture with the same weight as part A. The weight percent of the NdFeB nanomagnets varies from 50, 75, and 83 wt% with the silicone polymer. After mixing thoroughly for 10 min to introduce microbubbles, the three-phase mixture was added to a syringe. Then, the syringe was put into a customized mechanical platform, in which the plunger was propelled by a linear motor (LinMot, H01-37). The soft magnetic fiber was extruded out under pressure of ~300 kPa via a nozzle. The extruded fiber was collected with a metal plate, which was then placed on a hotplate at 60 °C for 30 min (Fisher Scientific, Isotemp). Subsequently, the soft magnetic fiber was magnetized at impulse fields (2.65 T) by an impulse magnetizer (IM-10-30, ASC Scientific) with programmed directions. Soft magnetic fibers made by 83 wt% of SrFe12O19 (Sigma Aldrich) or Fe3O4 (Sigma Aldrich) are also fabricated with the same procedure as NdFeB.
4
Graphene-based Sensor Fabrication
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Graphene dispersion (1 mg/mL in DMF), graphene nanoplatelets (1 mg/mL, water dispersion), Fe3O4/graphene nanocomposite (10 mg/mL acetone dispersion, Fe3O4 NPs size: 5–25 nm), CoPt/graphene nanocomposite (10 mg/mL acetone dispersion, CoPt NPs size: 2–5 nm), and TiO2/graphene nanocomposite (10 mg/mL, acetone dispersion, TiO2 NPs size: 10–40 nm) have been purchased from Sigma Aldrich and used as received.
The size of silver interdigitates electrodes on alumina substrate was 7 mm × 13.4 mm. The interdigitates contacts consisted of 7 + 7 branches and each silver line has a 210 μm width.Figure 1 a report the picture of an interdigitated substrate.
In order to prepare the sensors, 15 droplets of each graphene-based solution have been drop-casted on 5 interdigitated alumina substrates and let dry upon solvent evaporation at room temperature. The prepared samples will be labelled as Gr dispersion, Gr_nanoplatelets, Gr_Fe3O4, Gr_CoPt, and Gr_TiO2.
The size of silver interdigitates electrodes on alumina substrate was 7 mm × 13.4 mm. The interdigitates contacts consisted of 7 + 7 branches and each silver line has a 210 μm width.
In order to prepare the sensors, 15 droplets of each graphene-based solution have been drop-casted on 5 interdigitated alumina substrates and let dry upon solvent evaporation at room temperature. The prepared samples will be labelled as Gr dispersion, Gr_nanoplatelets, Gr_Fe3O4, Gr_CoPt, and Gr_TiO2.
5
Synthesis and Characterization of TBBPA-Magnetite Composites
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4,4′-isopropylidenebis(2,6-dibromophenol) (tetrabromobisphenol A, TBBPA) (97%) was purchased from Alfa Aesar (Haverhill, MA, USA), tert-butanol (t-BuOH) (Honeywell/Riedel-de-Haen, Seelze, Germany). Magnetites were obtained from Sigma-Aldrich: Fe3O4 (F1), characterise by particle diameter of ~5 µm, a density of 4.8–5.1 g cm−3 and 95% trace metals while, the second samples of magnetite nanopowder (F2) was characterised by a diameter of less than 50 nm, a density of 4.8–5.1 g cm−3 and >98% trace metals. All chemicals used in these experiments were of analytical grade and were used without additional purification.
6
Magnetic Microbubble Preparation Protocol
7
Chitosan-Based Nanocomposite Characterization
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CS of high viscosity (>400 mPa.s) was purchased from Sigma-Aldrich (Saint Louis, MS, USA). Its degree of deacetylation (DDA = 86%) was determined from FTIR spectroscopy measurement [27 (link)], and its molecular weight (Mw = 1300 kDa) was obtained from viscosity measurements using the Mark–Houwink relationship [28 ] (i.e., the intrinsic viscosity is 0.992 mL/mg). Acetic acid, pentasodium tripolyphosphate (TPP), hydrochloric acid (HCl), ethanol, aspirin (ASA), and the necessary reagents to prepare the required buffer solutions (Na2HPO4 and NaH2PO4) were purchased from Sigma-Aldrich. The used NPs TiO2 (21 nm size), Fe3O4 (<50 nm size), and Al2O3 (<50 nm size) were also purchased from Sigma-Aldrich.
Two simulated fluids were prepared for the swelling test and the ASA transport experiments. The SGF solution (simulating gastric fluid), with a pH of 1.2, was a solution of 0.06M of HCl. The SIF solution (simulating intestinal fluid without enzymes), with a pH of 6.8, was prepared by mixing 46.3 mL of Na2HPO4 (1M) and 53.7 mL of NaH2PO4 (1M), adjusted up to 1 L with distilled water.
Two simulated fluids were prepared for the swelling test and the ASA transport experiments. The SGF solution (simulating gastric fluid), with a pH of 1.2, was a solution of 0.06M of HCl. The SIF solution (simulating intestinal fluid without enzymes), with a pH of 6.8, was prepared by mixing 46.3 mL of Na2HPO4 (1M) and 53.7 mL of NaH2PO4 (1M), adjusted up to 1 L with distilled water.
8
Forensic Analysis of Car Paint Chips
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Three car paint chips (samples A to C as shown in Figure 1 a–c, respectively) were provided by the Forensic Science Laboratory of Hyogo Prefectural Police Headquarters (Hyogo, Japan). The numbers of layers of samples A to C were confirmed to be 9, 5, and 4, respectively, by optical, SEM, and ATR-FTIR imaging (Figure 2 ). Reagent grade chemicals used as standard reference materials for ATR-FTIR imaging and RMS measurements, such as rutile (TiO2), anatase (TiO2), BaSO4, ZnO, Zn3(PO4)2, Fe3O4, and CaCO3, were purchased from Sigma Aldrich (Darmstadt, Germany). Standard minerals, such as kaolinite (KGa-1b), were obtained from the International Clay Mineral Society (ICMS), while talc and pyrophyllite were collected from the Korea Institute of Geoscience and Mineral Resources (KIGAM).
9
Magnetic A5 Protein Wire Synthesis
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Using the
methods above (formation
of theA5 wire, followed by cross-linking of the wire),
75 μL (10 mg/mL)A5 and 25 μL (5 mg/mL) iron
nanoparticles (Fe3O4, 5 nm, Sigma-Aldrich),
were mixed to form anA5 -wire containing the magnetic
nanoparticles. The cross-linked wire was confirmed to be magnetic
using a neodymium magnet.
methods above (formation
of the
75 μL (10 mg/mL)
nanoparticles (Fe3O4, 5 nm, Sigma-Aldrich),
were mixed to form an
nanoparticles. The cross-linked wire was confirmed to be magnetic
using a neodymium magnet.
10
Isolation and Culture of Murine Microvascular PASMCs
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Murine microvascular PASMCs were isolated and cultured as described previously (Malczyk et al., 2013 (link)). Briefly, lungs were filled with 0.5% low-melting-point agarose (type VII, Sigma-Aldrich, #A9414) and 0.5% Fe3O4 (Sigma-Aldrich, #518158), mechanically shredded using scissors and digested with collagenase (80 U/ml, Sigma-Aldrich, #C0773) for 1 h. Then, tissue was washed in PBS (Capricorn Scientific, Ebsdorfergrund, Germany, #PBS-2A) using a magnet and PASMCs attached to microvascular pulmonary vessels were seeded using Smooth Muscle Basal Medium 2 (PromoCell, Madison, Wisconsin, United States, Cat# C-22262) containing 5% Smooth Muscle Basal Medium 2 Supplement (PromoCell, Cat# C-39267), 0.5% Normocin (Invivo-Gen, Toulouse, France) and 10% FCS (Sigma-Aldrich, St. Louis, Missouri, United States, Cat# F0804). It takes several hours up to 1 day until the PASMCs start outgrowing from the isolated precapillary pulmonary vessel pieces. During that time, the cells are already adherent. After 2–3 days, PASMCs are sufficiently grown to be used in experiments. Growing conditions can be normoxic or hypoxic from day 0.
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