The tumor tissues were thawed at room temperature and homogenized by bead beating in PBS (1 mL PBS/100 mg tissue) at 4 °C. After centrifuging at a speed of 15000 rpm/min for 10 min, the supernatants were collected for ELISA tests[30 (link)]. The procedures were followed the manufacturer’s instructions of TNF-α (Invitrogen, #KHC3011), IL-6 (Abcam, ab222503), IFN-β (Bio-techne, #42400-1) and IFN-γ (LSBio, LS-F3414) ELISA kits.
Ls f3414
Manufactured by LSBio
The LS-F3414 is a laboratory equipment designed for use in scientific research and analysis. It serves as a tool for measuring and analyzing various properties of samples. The core function of this product is to provide accurate and reliable data to support scientific investigations. No further details or interpretations are provided.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ls f3414
1
Measuring Cytokine Levels in Tumor Tissues
2
Tissue Homogenization and Immune Assays
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