Following incubation of 50 µg/ml rhβ2-GP I, aβ2-GP I and negative control IgG at 37°C for 24 h, all five groups (rhβ2-GP I, aβ2-GP I, IgG, aβ2-GP I/rhβ2-GP I and IgG/rhβ2-GP I) of JEG-3 cells were washed once with PBS, fixed with 70% ethanol at 4°C for 24 h, stained with propidium iodide/RNase staining buffer solution (BD Biosciences, San Jose, CA, USA) and analyzed with a flow cytometer (Beckman Coulter, Inc., Brea, CA, USA) and ModFit software 3.2.1 (Verity Software House, Inc., Topsham, ME, USA).
Propidium iodide rnase staining buffer solution
Manufactured by BD
Sourced in United States
Propidium iodide/RNase staining buffer solution is a laboratory reagent used for DNA content analysis and cell cycle studies. It contains propidium iodide, a fluorescent dye that binds to DNA, and RNase, an enzyme that removes cellular RNA. The solution is designed to stain and prepare cells for analysis using flow cytometry or other fluorescence-based techniques.
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Lab products found in correlation
2 protocols using propidium iodide rnase staining buffer solution
1
Cell Cycle Analysis of JEG-3 Cells
2
Cell Cycle Analysis of CRC Cells
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CRC cell lines were treated with vehicle (0.1% DMSO in PBS), cisplatin (2 and 0.2 µg/ml), NAM (3 and 5 mM) or a combination of these drugs for 72 h at 37˚C. The treated cells were fixed in 70% ethanol for at least 12 h at -20˚C, followed by incubation with 500 µl propidium iodide/RNase Staining Buffer Solution (BD Pharmingen; BD Biosciences) for 15 min at room temperature. The stained cells were assessed by flow cytometry using a FACSMelody Flow Cytometer (BD Biosciences) and the proportion of cells in the different cell cycle stages were analyzed using the Modfit LT software (version 3.1; Verity Software House).
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