The largest database of trusted experimental protocols

Carbon coated copper grids

Manufactured by Science Services
Sourced in Germany

Carbon-coated copper grids are a type of lab equipment used for sample preparation in electron microscopy. They provide a stable and conductive substrate for mounting and imaging samples using transmission electron microscopes (TEMs) or scanning electron microscopes (SEMs).

Automatically generated - may contain errors

2 protocols using carbon coated copper grids

1

Magnetosome Biosynthesis Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Growth of Tn5 insertants was assessed by measuring optical density (OD) at 565 nm. Cultures with no severe growth defect were further screened for magnetic phenotype by determination of cmag (36 (link)). For a set of mutants, cells were also assessed by optical microscopy for their swimming behavior, cell shape, and alignment in response to an externally applied magnetic field. Magnetosome morphology was analyzed with respect to size, shape, and numbers per cell by TEM analysis. For this, concentrated cells were adsorbed onto carbon-coated copper grids (Science Services, Munich, Germany) and imaged at 80 kV without negative staining in a TECNAI F20 microscope (FEI, Eindhoven, Netherlands). Ultrastructural analysis of mutants provided information on modifications in magnetosome biosynthesis as well as magnetosome organization.
+ Open protocol
+ Expand
2

Quantifying Magnetite Biomineralization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Transmission electron microscopy of whole cells was performed with specimens directly deposited onto carbon-coated copper grids (Science Services, Munich, Germany). For TEM imaging a Jeol Jem 1400 + (Freising, Germany) was operated at an acceleration voltage of 80 kV. Image acquisition was performed with a Gatan Erlangshen ES500W CCD camera. Average particle sizes were measured by data processing with ImageJ software package v1.52i. For quantitative assessment of magnetite particle biomineralization at different timepoints, specifically at the process start, in the mid-exponential growth phase and at end of growth 1000 magnetosome particles per triplicate were counted and combined for evaluation.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!