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Propofol

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Propofol is an intravenous anesthetic agent used to induce and maintain anesthesia. It is a clear, colorless, and lipophilic liquid that is formulated for injection. Propofol acts on the central nervous system to produce a rapid onset of unconsciousness and a short duration of action.

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Lab products found in correlation

Propofol, by Steraloids (3 mentions) Cholesterol, by Steraloids (2 mentions) Fullerene c60, by Merck Group (1 mentions) Carbon black, by Merck Group (1 mentions) β estradiol 3 β d glucuronide sodium salt, by Merck Group (1 mentions) Serotonin β dglucuronide, by LGC (1 mentions) Serotonin d4 β d glucuronide, by LGC (1 mentions) Serotonin hydrochloride, by Merck Group (1 mentions) β estradiol, by Merck Group (1 mentions) Imipramine hydrochloride, by Merck Group (1 mentions) Alamethicin, by Merck Group (1 mentions)

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Propofol-O-glucuronide (5 citations)

5 protocols using propofol

1

Propofol Effect on Cholesterol Metabolism

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The effect of propofol (Toronto Research Chemicals) on cholesterol hydroxylation was tested as described by Mast et al20 using 46 μM propofol and 2.3 μM cholesterol, the concentrations used to identify CYP27A1 inhibitors. To evaluate the effect of propofol on 5β‐cholestane‐3α,7α,12α‐triol conversion to 5β‐cholestane‐3α,7α,12α,27‐tetrol, the same experimental set‐up was used, using 0.01 μM human recombinant CYP27A1, 3.0 μM adrenodoxin, 0.05 μM adrenodoxin reductase (EC 1.18.1.6), and 2.3 μM 5β‐cholestane‐3α,7α,12α‐triol (Steraloids). Human recombinant CYP27A1, bovine adrenodoxin, and bovine adrenodoxin reductase were expressed and purified as described.22, 23, 24 The assay for IC50 determination used propofol concentrations up to 1 mM for both cholesterol hydroxylation and 5β‐cholestane‐3α,7α,12α‐triol conversion.
Claesen J.L., Koomen E., Schene I.F., Jans J.J., Mast N., Pikuleva I.A., van der Ham M., de Sain‐van der Velden M.G, & Fuchs S.A. (2020). Misdiagnosis of CTX due to propofol: The interference of total intravenous propofol anaesthesia with bile acid profiling. Journal of Inherited Metabolic Disease, 43(4), 843-851.
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2

Propofol's Impact on Cholesterol Metabolism

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The effect of propofol (Toronto Research Chemicals) on cholesterol hydroxylation was tested as described by Mast et al. (2015) (20 (link)) using 46 μM propofol and 2.3 μM cholesterol, the concentrations used to identify CYP27A1 inhibitors. To evaluate the effect of propofol on 5β-cholestane-3α,7α,12α-triol conversion to 5β-cholestane-3α,7α,12α,27-tetrol, the same experimental set-up was used, using 0.01 μM human recombinant CYP27A1, 3.0 μM adrenodoxin, 0.05 μM adrenodoxin reductase (EC 1.18.1.6) and 2.3 μM 5β-cholestane-3α,7α,12α-triol (Steraloids). Human recombinant CYP27A1, bovine adrenodoxin, and bovine adrenodoxin reductase were expressed and purified as described (22 –24 (link)). The assay for IC50 determination used propofol concentrations up to 1 mM for both cholesterol hydroxylation and 5β-cholestane-3α,7α,12α-triol conversion.
Claesen J.L., Koomen E., Schene I.F., Jans J.J., Mast N., Pikuleva I.A., van der Ham M., de Sain‐van der Velden M.G, & Fuchs S.A. (2020). Misdiagnosis of CTX due to propofol: The interference of total intravenous propofol anaesthesia with bile acid profiling. Journal of Inherited Metabolic Disease, 43(4), 843-851.
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3

Recombinant CYP27A1 Ligand Binding

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Human recombinant CYP27A1 was expressed and purified as described by Mast et al. (2006) (22 ). Propofol (Toronto Research Chemicals Inc.) and cholesterol (Steraloids Inc.) binding to CYP27A1 was analysed in vitro according to the method described by Lam et al. (2018) (19 (link)), using Propofol concentrations of 20, 40 and 100 μM and cholesterol concentrations up to 3.0 μM.
Claesen J.L., Koomen E., Schene I.F., Jans J.J., Mast N., Pikuleva I.A., van der Ham M., de Sain‐van der Velden M.G, & Fuchs S.A. (2020). Misdiagnosis of CTX due to propofol: The interference of total intravenous propofol anaesthesia with bile acid profiling. Journal of Inherited Metabolic Disease, 43(4), 843-851.
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4

Characterizing Propofol and Cholesterol Binding to CYP27A1

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Human recombinant CYP27A1 was expressed and purified as described by Mast et al.22 Propofol (Toronto Research Chemicals, Inc) and cholesterol (Steraloids, Inc) binding to CYP27A1 was analysed in vitro according to the method described by Lam et al,19 using Propofol concentrations of 20, 40, and 100 μM and cholesterol concentrations up to 3.0 μM.
Claesen J.L., Koomen E., Schene I.F., Jans J.J., Mast N., Pikuleva I.A., van der Ham M., de Sain‐van der Velden M.G, & Fuchs S.A. (2020). Misdiagnosis of CTX due to propofol: The interference of total intravenous propofol anaesthesia with bile acid profiling. Journal of Inherited Metabolic Disease, 43(4), 843-851.
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5

Glucuronidation of Pharmaceuticals and Metabolites

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Chemicals EC1.5-P-and FH-P-SWCNTs were obtained from Meijo Nano Carbon Co., Ltd. (Nagoya, Japan). Alamethicin, β-estradiol, β-estradiol 3-(β-D-glucuronide) sodium salt, carbon black, fullerene-C 60 , and fullerene-C 70 , imipramine hydrochloride, serotonin hydrochloride, and uridine 5ʹ-diphosphoglucuronic acid (UDPGA) trisodium salt were purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.). Imipramine N-β-D-glucuronide, propofol, serotonin β-Dglucuronide, and serotonin-d4 β-D-glucuronide were obtained from Toronto Research Chemicals (Toronto, Canada). Furthermore, propofol β-D-glucuronide was acquired from the Cayman Chemical Company (Ann Arbor, MI, U.S.A.).
Asai Y., Nadai M, & Katoh M. (2022). The Effect of Single-Walled Carbon Nanotubes on UDP-Glucuronosyltransferase 1A Activity in Human Liver. Biological & pharmaceutical bulletin, 45(4).
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