Ab59721

Manufactured by Abcam

Ab59721 is a primary antibody that recognizes the Apolipoprotein B (APOB) protein. It is suitable for use in various immunoassays, including Western blotting and Immunohistochemistry applications.

Automatically generated - may contain errors

Lab products found in correlation

Ab54966, by Abcam (1 mentions) Hpa005993, by Merck Group (1 mentions) Hpa023923, by Merck Group (1 mentions) Envision kit, by Agilent Technologies (1 mentions) Ab113509, by Abcam (1 mentions) Ab84903, by Abcam (1 mentions) Pa5 24634, by Thermo Fisher Scientific (1 mentions) Ferrostatin 1, by Selleck Chemicals (1 mentions) Hrp labeled secondary antibody conjugates, by Thermo Fisher Scientific (1 mentions) Necrosulfonamide ab143839, by Abcam (1 mentions) Z vad fmk, by Abcam (1 mentions) Erastin, by Selleck Chemicals (1 mentions)

2 protocols using ab59721

Immunohistochemical Analysis of Cancer Markers

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Consecutive sections (4-μm) of tissue microarray samples were mounted on the APES-coated slides. After de-paraffinization, rehydration and antigen retrieval using an autoclave-oven-technique, sections were incubated with anti-PRR11 (HPA023923, SIGMA-ALDRICH), UCHL1 (HPA005993, SIGMA-ALDRICH), SNAT1 (ab59721, abcam®), and EGR1 (ab54966, abcam®) at 4°C overnight. A two-step EnVisionTM KIT (DAKO, USA) was used to visualize positive staining. Lung cancers known to be positive for these proteins were used as positive controls. Replacement of the primary antibody by PBS served as a negative control.

Chen Y., Cha Z., Fang W., Qian B., Yu W., Li W., Yu G, & Gao Y. (2015). The prognostic potential and oncogenic effects of PRR11 expression in hilar cholangiocarcinoma. Oncotarget, 6(24), 20419-20433.

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Antibody-Based Protein Detection Protocol

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The following antibodies were used in this study: SLC1A5 (ab84903, Abcam), glutaminase 2 (GLS2; ab113509, Abcam), GOT1 (PA5-24634, Thermo), SLC38A1 (ab59721, Abcam), Flag (F3165, clone M2; SigmaAldrich), Actin (sc-8432, Santa Cruz). HRP-labeled secondary antibody conjugates were purchased from Molecular Probes (Thermo). Erastin (#E7781), RSL3 (#S8155), and ferrostatin-1 (#S7243) were obtained from Selleck Chemicals (Houston, TX, USA). Necrosulfonamide (ab143839) and Z-VAD-FMK (ab120382) were obtained from Abcam. Compound 968 (352010), GPNA (G1135), and amino-oxyacetate (AOA; C13408) were obtained from Sigma.

Luo M., Wu L., Zhang K., Wang H., Zhang T., Gutierrez L., O’Connell D., Zhang P., Li Y., Gao T., Ren W, & Yang Y. (2018). miR-137 regulates ferroptosis by targeting glutamine transporter SLC1A5 in melanoma. Cell Death and Differentiation, 25(8), 1457-1472.

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