Lentiviral shrna pgipz vectors ns rhs4348

Manufactured by Thermo Fisher Scientific

The Lentiviral shRNA pGIPZ vectors; NS (RHS4348) are a set of plasmid constructs designed for the expression of short hairpin RNA (shRNA) sequences in mammalian cells. These vectors contain the necessary elements for the production of lentiviral particles, which can be used to stably transduce and express the shRNA in the target cells.

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Lab products found in correlation

Ex neg m02, by GeneCopoeia (3 mentions) Ex z0396 m02, by GeneCopoeia (2 mentions) αcd133 v2lhs 71816, by Thermo Fisher Scientific (2 mentions) Puromycin, by Takara Bio (1 mentions) Geneticin, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Lentiviral pGIPZ vectors PGIPZ lentiviral vector Lentiviral shRNA vectors PGIPZ-Ns PGIPZ vector Lentiviral vector RHS4348 PGIPZ

3 protocols using lentiviral shrna pgipz vectors ns rhs4348

CD133 Expression Plasmid and shRNA

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Human cDNA CD133 expression plasmid (EX-Z0396-M02) and empty vector plasmid (EX-NEG-M02) were obtained from GeneCopoeia. Lentiviral shRNA pGIPZ vectors; NS (RHS4348) and αCD133 (V2LHS_71816) were obtained from Thermo Scientific. IKK (IKK-2 S177E S191E) and IKBα (pBabe-Puro-IKBalpha-mut (superrepressor)) plasmids were obtained from Addgene.

Nomura A., Banerjee S., Chugh R., Dudeja V., Yamamoto M., Vickers S.M, & Saluja A.K. (2015). CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer. Oncotarget, 6(10), 8313-8322.

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Modulating NF-κB Activity in Cells

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Human cDNA empty vector plasmid (EX-NEG-M02) and CD133 expression plasmid (EX-Z0396-M02) were attained from GeneCopoeia. Lentiviral shRNA pGIPZ vectors; NS (RHS4348) and αCD133 (V2LHS_71816) were acquired from Thermo Scientific. NF-kB activity was inhibited after transfecting cells with pBabe-puro-IKBalphamut suprerepressor (a gift from William Hahn [ Addgene plasmid#15291]. In this plasmid, the IκBα is mutated at S32A, which keeps NF-κB pathway constitutively repressed (33 (link)).

Nomura A., Gupta V.K., Dauer P., Sharma N.S., Dudeja V., Merchant N., Saluja A.K, & Banerjee S. (2017). NFkB-mediated Invasiveness in CD133+ Pancreatic TICs is Regulated by Autocrine and Paracrine Activation of IL-1 Signaling. Molecular cancer research : MCR, 16(1), 162-172.

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Establishing Stable Cell Lines for CD133 Expression

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Human cDNA CD133 expression plasmid (EX-Z0396-M02) and empty vector plasmid (EX-NEG-M02) were obtained from GeneCopoeia. Lentiviral shRNA pGIPZ vectors; NS (RHS4348) and aCD133 (V2LHS_71816) were obtained from Thermo Scientific.
MIA PaCa-2 (ATCC) and stable MIA-derivatives were maintained in DMEM (Hyclone) containing 10% fetal bovine serum. S2-VP10 cells were cultured in RPMI 1640 (Hyclone) supplemented with 10% fetal bovine serum. Stable clones were selected and maintained in Geneticin (Invitrogen) and Puromycin (Clontech) for MIA PaCa-2 and S2-VP10 derivatives, respectively.

Nomura A., Dauer P., Gupta V., McGinn O., Arora N., Majumdar K., III C.U., Dalluge J., Dudeja V., Saluja A, & Banerjee S. (2016). Microenvironment mediated alterations to metabolic pathways confer increased chemo-resistance in CD133+ tumor initiating cells. Oncotarget, 7(35), 56324-56337.

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