Bot 64

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The BOT-64 is a laboratory instrument designed for high-throughput cell culture and sample processing. It features a compact, automated system that can handle a variety of liquid handling tasks, including cell seeding, media changes, and compound addition. The BOT-64 is capable of working with 96-well, 384-well, and 1536-well microplates, facilitating efficient and reproducible experimental workflows.

Automatically generated - may contain errors

Lab products found in correlation

Nsc95397, by Merck Group (1 mentions) Sr11302, by Santa Cruz Biotechnology (1 mentions) Tpca 1, by Merck Group (1 mentions) T 5224, by GLPBIO (1 mentions) Mg132, by Merck Group (1 mentions) Tpca 1, by Abcam (1 mentions) Il 6 406 ml, by R&D Systems (1 mentions) Bay 11 7082, by Abcam (1 mentions)

2 protocols using bot 64

Investigating NF-κB and AP-1 Signaling in IL-1β Response

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Cells were treated with two NF-κB inhibitors, TPCA1 (5 μM, Sigma-Aldrich, #T1452), and BOT-64 (5 μM, Santa Cruz Biotechnology, #sc-222062), and two AP-1 inhibitors, T-5224 (50 μM, GLPBIO, Montclair, CA, USA, #GC16165) and SR11302 (50 μM, Santa Cruz Biotechnology, #sc-204295), for 4 h and then stimulated with 50 ng/mL recombinant IL-1β for 2 h. To block CtBP2-mediated signaling, cells were treated with two CtBP inhibitors, MTOB (5 μM, MedKoo Biosciences, Morrisville, NC, USA, #561414) and NSC95397 (5 μM, Sigma-Aldrich, #N1786). In addition, cells were also only treated with different concentrations (0, 5, 50 and 500 ng/mL) of IL-1β. The resulting cells were applied to RNA or protein isolation to detect gene expression or protein level changes.

Zhou P., Wan X., Zou Y., Chen Z, & Zhong A. (2020). Transforming growth factor beta (TGF-β) is activated by the CtBP2-p300-AP1 transcriptional complex in chronic renal failure. International Journal of Biological Sciences, 16(2), 204-215.

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Recombinant Proteins and Inhibitor Reagents for Cell Signaling

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Recombinant proteins were purchased from R&D Systems: EDA-A1 (3944-ED), EDA-A2 (922-ED), TNFα (410-MT), BAFF (8876-BF), TWEAK (1237-TW), OSM (495-MO), IL-6 (406-ML) and LIF (8878-LF). Small molecule inhibitors were acquired from indicated sources: TPCA-1 (abcam, ab145522), MG132 (Sigma; M8699), BAY 11-7082 (abcam, ab141228), BOT-64 (Santa Cruz; sc-222062), B022 (Aobious; AOB8699). Mouse EDA-A1 (MC208411), mouse EDA-A2 (MC208415) and mouse OSM (MR226014) expression plasmids were purchased from Origene.
Mouse NIK expression plasmid was purchased from Invivogen (pUNO1-mMap3k14). Wild-type human NIK and the NIK-K429/430A mutant plasmids were a gift from Prof. Michael Kracht (JLU Giessen).

Bilgic S.N., Domaniku A., Toledo B., Agca S., Weber B.Z.C., Arabaci D.H., Ozornek Z., Lause P., Thissen J.P., Loumaye A, & Kir S. (2023). EDA2R-NIK signalling promotes muscle atrophy linked to cancer cachexia. Nature, 617(7962).

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