Polyethylene glycol methyl ether (MPEG, Mn = 2000, Fluka, USA), ε-caprolactone (ε-CL, Alfa Aesar, USA), stannous octoate (Sn(Oct)2, Sigma, USA), doxorubicin (DOX, Sigma), methyl thiazolyl tetrazolium (MTT, Sigma, USA), Dulbecco’s modified eagle medium (DMEM Gibco, USA), fetal bovine serum (FBS, Gibco, USA), alginate sodium (Sigma, USA), fluorescein isothiocyanate-dextran (FITC-dextran, Sigma, USA), Annexin V-FITC/PI Detection kit (keyGEN Biotech, China), tricaine (Sigma, USA), 1-phyenyl-2-thiourea (PTU, Sigma, USA), and methanol (HPLC grade, Fisher scientific, UK) were used without further purification. HK was purchased from Suzhou NeuPharma Co. All the materials used in this article were analytic reagent (AR) grade and used as received.
C6 glioma cells were maintained and grown in DMEM supplemented with 10% FBS in a 37 °C incubator with a humidified 5% CO2 atomosphere.
Tg(flk:EGFP) transgenic zebrafish were provided by Dr. Shuo Lin (UCLA, USA). Female BALB/c nude mice (6–8 weeks old) were purchased from the Laboratory Animal Center of Sichuan University (Chengdu, China). Animal experiments were performed according to the guidelines of the Animal Care and Use Committee of Sichuan University (Chengdu, Sichuan, China) and approved by the Animal Care and Use Committee of Sichuan University.
C6 glioma cells were maintained and grown in DMEM supplemented with 10% FBS in a 37 °C incubator with a humidified 5% CO2 atomosphere.
Tg(flk:EGFP) transgenic zebrafish were provided by Dr. Shuo Lin (UCLA, USA). Female BALB/c nude mice (6–8 weeks old) were purchased from the Laboratory Animal Center of Sichuan University (Chengdu, China). Animal experiments were performed according to the guidelines of the Animal Care and Use Committee of Sichuan University (Chengdu, Sichuan, China) and approved by the Animal Care and Use Committee of Sichuan University.