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Mabpac sec 1 analytical column

Manufactured by Thermo Fisher Scientific
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The MAbPac SEC-1 analytical column is designed for the size-exclusion chromatographic separation of monoclonal antibodies and other large biomolecules. It features a silica-based stationary phase with a pore size optimized for the analysis of these types of samples.

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MAbPac SEC-1 column MAbPac SEC-1 Analytical columns

3 protocols using mabpac sec 1 analytical column

1

Long-term Storage Stability of ADCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Each ADC (1 mg/mL, 10 μL) in PBS was incubated at 37°C for 28 days and stored at −80°C until use. Samples were analyzed using an Agilent 1100 HPLC system equipped with a MAbPac SEC-1 analytical column (4.0 × 300 mm, 5 μm, Thermo Scientific). The conditions were as follows: flow rate = 0.2 mL/min; solvent = PBS. All assays were performed in triplicate.
Anami Y., Otani Y., Xiong W., Ha S.Y., Yamaguchi A., Rivera-Caraballo K.A., Zhang N., An Z., Kaur B, & Tsuchikama K. (2022). Homogeneity of antibody-drug conjugates critically impacts the therapeutic efficacy in brain tumors. Cell reports, 39(8), 110839.
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2

Stability Monitoring of Antibody-Drug Conjugates

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Each ADC (1 mg/mL, 100 μL) in PBS was incubated at 37 °C.
An aliquot (10 μL) was taken after 28 days and analyzed using an Agilent
1100 HPLC system equipped with a MAbPac SEC-1 analytical column (4.0×300
mm, 5 μm, Thermo Scientific). The conditions were as follows: solvent =
PBS; flow rate = 0.2 mL/min.
Anami Y., Deng M., Gui X., Yamaguchi A., Yamazaki C.M., Zhang N., Zhang C.C., An Z, & Tsuchikama K. (2020). LILRB4-Targeting Antibody–Drug Conjugates for the Treatment of Acute Myeloid Leukemia. Molecular cancer therapeutics, 19(11), 2330-2339.
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3

Long-term Storage Stability of ADCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Each ADC (1 mg/mL, 10 μL) in PBS was incubated at 37°C for 28 days and stored at −80°C until use. Samples were analyzed using an Agilent 1100 HPLC system equipped with a MAbPac SEC-1 analytical column (4.0 × 300 mm, 5 μm, Thermo Scientific). The conditions were as follows: flow rate = 0.2 mL/min; solvent = PBS. All assays were performed in triplicate.
Anami Y., Otani Y., Xiong W., Ha S.Y., Yamaguchi A., Rivera-Caraballo K.A., Zhang N., An Z., Kaur B, & Tsuchikama K. (2022). Homogeneity of antibody-drug conjugates critically impacts the therapeutic efficacy in brain tumors. Cell reports, 39(8), 110839.
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