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Cd31 fitc

Manufactured by Abcam
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CD31-FITC is a fluorescently labeled antibody that binds to the CD31 (PECAM-1) protein. CD31 is a cell adhesion molecule expressed on the surface of endothelial cells, platelets, and certain immune cells. The FITC (fluorescein isothiocyanate) label allows for the detection and visualization of CD31-positive cells in various applications.

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Lab products found in correlation

L nna, by Merck Group (1 mentions) Anti rat cd3 pe, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

FITC conjugated anti-CD31 antibody (2 citations) FITC anti-CD31 (2 citations)

2 protocols using cd31 fitc

1

Phenotypic Characterization of Cells

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For phenotypic characterization, cells were stained with fluorochrome-conjugated monoclonal antibodies including CD45-FITC (Elabscience, E-AB-F1227C), CD31-FITC (Abcam, ab33858), CD90-FITC (Elabscience, E-AB-F1226C) and CD44-FITC (Elabscience, E-AB-F1225C). Corresponding mouse IgG1-FITC (Elabscience, E-AB-F09792C) and mouse IgG2a-FITC (Elabscience, E-AB-F09802C) were used as isotype control. The samples were incubated for 30 min in the dark and then washed for 2 times. All samples were resuspended in 500 μl PBS and analyzed by flow cytometer (Beckman, CytoFLEX LX) and FlowJo software (Version 10.8.1, Tree Star, San Carlos, CA).
Li M., Yu Y., Xue K., Li J., Son G., Wang J., Qian W., Wang S., Zheng J., Yang C, & Ge J. (2023). Genistein mitigates senescence of bone marrow mesenchymal stem cells via ERRα-mediated mitochondrial biogenesis and mitophagy in ovariectomized rats. Redox Biology, 61, 102649.
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2

Rat Endothelial Cell Isolation

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L-NNA was purchased from sigma (St. Louis, MO, USA). Anti-Rat CD54 PerCP-eFluor 710 and Anti-Rat CD3PE were purchased from eBioscience (San Diego, California, USA). CD31 FITC and rabbit monoclonal antibody to CD146 were purchased from Abcam (London, UK). CD62P PerCP was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Uncaria rhynchophylla and Semen Raphani were purchased from Jianlian Traditional Chinese medicine Store (Jinan, China).
Li Y., Yang W., Zhu Q., Yang J, & Wang Z. (2015). Protective effects on vascular endothelial cell in N'-nitro-L-arginine (L-NNA)-induced hypertensive rats from the combination of effective components of Uncaria rhynchophylla and Semen Raphani. Bioscience trends, 9(4).
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