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Bromocresol purple

Manufactured by Thermo Fisher Scientific
Sourced in United States

Bromocresol purple is a pH indicator dye commonly used in analytical laboratory procedures. It exhibits a color change from yellow to purple over the pH range of 5.2 to 6.8, making it useful for monitoring pH levels in various applications.

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2 protocols using bromocresol purple

1

Fluorescent Bacterial Viability Assay

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Poly-L-lysine (PLL) (average molecular weight: 4700) was kindly provided by JNC corporation (Tokyo, Japan) as a gift. Sodium chloride and L-cysteine were purchased from Sigma-Aldrich (St. Louis, MO, USA). BactoTM Lactobacilli MRS Broth (MRS) and agar were purchased from BD biosciences (San Jose, CA, USA). Bromocresol purple, ciprofloxacin and LIVE/DEADTM BaclightTM Bacterial Viability Kit were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Flamma® 648 NHS ester was purchased from BioActs (Incheon, Korea). All other materials and solvents used were of the highest analytical grade.
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2

Sucrose Utilization Assay with Antimycin A

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The yeast strains were grown as liquid cultures in synthetic complete (SC)-Leu at 30° C to mid-log phase, then diluted to an OD600 of 0.5. Serial dilutions were pinned using a 48-pin manifold onto 2% sucrose indicator solid media23 (link) containing bromocresol purple (Thermo-Fisher Scientific), in the absence or presence of 10 μg/mL antimycin A (Fisher Scientific). The BP (bromocresol purple) indicator plates were grown at 30° C for two days before imaging.
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