Scanjet g4010

The degree of casein hydrolysis in the cheeses during ripening was monitored using freeze‐dried water‐insoluble fractions of the cheeses and urea‐polyacrylamide gel electrophoresis (Urea‐PAGE) instrument (Protean II XI vertical slab gel unit, Bio‐Rad Laboratories Ltd., Watford, UK) according to the method described by Andrews (1983 (link)). Staining of the obtained gels was accomplished directly by the method of Blakesley and Boezi (1977 (link)) with Coomassie Brilliant Blue G‐250. Next, the gels were destained by pure water, and gel slabs were digitized using a scanner (HP ScanJet software, ScanJet G4010, Hewlett Packard, Palo Alto, CA).

Whole embryo images were acquired using a Nikon SMZ1500 stereomicroscope. Images of peripheral blood and tissue sections were acquired using a Nikon Eclipse E600 microscope equipped with a Nikon DS-Fi3 microscope camera and NIS-Elements Documentation software (Nikon Instruments). Embryos were examined with a 0.5X objective; smears with a 100X/1.4 oil-immersion objective; and tissue sections with a 20X/0.60 or 40X/0.75 objectives. Agarose gel thermal images and western blot films were scanned using a HP Scanjet G4010 scanner and HP Easy Scan software v.1.8 (Hewlett Packard). Final images were assembled using Microsoft PowerPoint for Mac v.16 (Microsoft).