After the run, slides were removed from the Xenium Analyzer instrument and had quencher removed according to 10X Genomics’ Demonstrated Protocol CG000613. Immediately following, the slides were H&E stained according to the following protocol: xylene (x3, 3min ea), 100% alcohol (x2, 2min ea), 95% alcohol (x2, 2min ea), 70% alcohol (2min), DI water rinse (1min), hematoxylin (1min) (Biocare Medical CATHE), DI water rinse (1min), bluing solution (1min) (Biocare Medical HTBLU-M), DI water rinse (3min), 95% alcohol (30sec), eosin (5sec) (Biocare Medical HTE-GL), 95% alcohol (10sec), 100% alcohol (x2, 10sec ea), xylene (x2, 10sec ea). Coverslipping was performed using Micromount (Leica 3801731) and cured overnight at room temperature. Histology images taken on a 20X Leica Biosystems Aperio CS2.
Cathe
Manufactured by Biocare Medical
CATHE is a versatile laboratory instrument designed for automated cell analysis and counting. It utilizes advanced optical techniques to accurately measure and quantify various cell properties, including cell size, cell count, and cell viability. CATHE provides reliable and consistent results, supporting researchers and clinicians in their analytical workflows.
Automatically generated - may contain errors
Lab products found in correlation
Aperio cs2, by Leica Biosystems (1 mentions)
Cytokeratin, by Biocare Medical (1 mentions)
Vimentin, by Biocare Medical (1 mentions)
Diva decloaker solution, by Biocare Medical (1 mentions)
Peroxidazed 1 px968, by Biocare Medical (1 mentions)
Decloaking chamber, by Biocare Medical (1 mentions)
Intellipath autostainer, by Biocare Medical (1 mentions)
2 protocols using cathe
1
Xenium Analyzer Tissue Staining Protocol
2
Immunohistochemical Tumor Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Histological sections of tumor tissues were analyzed following H&E staining and additional immunostaining on selected sections to determine cell origins. IHC was performed on 4 μm slides of formalin-fixed and paraffin-embedded tumor tissue specimens. The sections were deparaffinized with changes of xylenes and graded ethanols to water. Antigen retrieval was performed in a Decloaking Chamber (Biocare) with Diva DeCloaker solution (Biocare Medical, DV 2004). Staining procedures were performed on a Biocare IntelliPath autostainer. Blocking was performed using Peroxidazed 1 (PX968, Biocare Medical) for 5 min, and Background Punisher (BP974, Biocare Medical) for 10 min. Sections were then incubated for 30 min at room temperature with a prediluted monoclonal antibody (Vimentin, SM Actin, MS Actin or Cytokeratin; Biocare Medical). Secondary antibody, mouse on canine polymer (Biocare Medical, MC541) was applied for 30 min. Finally, the chromagen, diaminobenzidine (DAB) was incubated for 5 min (IP FLX DAB, IPK5010, Biocare Medical) and followed by hematoxylin counterstain (CATHE, Biocare Medical) for 2 min. Negative controls were obtained by replacing the primary antibody with Polymer negative control serum (Biocare Medical, NC499) for 30 min.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!