All signal intensities of Western blotting images were analysed using imaging software (Image Gauge v4.21; Fujifilm) with the default manufacturer’s procedure. Briefly, the same size of pixel area was selected and signal intensity calculated by subtraction of the background signal. Each signal was normalised with reference to standard control signals, e.g. tubulin, and a signal to control ratio was calculated.
Image gauge v4
Manufactured by Fujifilm
Sourced in Japan
Image Gauge V4.0 is a software package for image measurement and analysis developed by Fujifilm. It provides tools for accurate dimensional measurements, shape analysis, and data reporting within digital images.
Automatically generated - may contain errors
Lab products found in correlation
Fuji fla 3000 phosphoimager, by Fujifilm (2 mentions)
Diff quick, by Sysmex (1 mentions)
Typhoon fla 9500, by Fujifilm (1 mentions)
Prism v6, by GraphPad (1 mentions)
Fla 5000 imaging analyzer, by Fujifilm (1 mentions)
Image lab v5, by Bio-Rad (1 mentions)
Enhanced chemical luminescence, by Cytiva (1 mentions)
Anti lamin a c ab, by Santa Cruz Biotechnology (1 mentions)
Las 3000 imager, by Fujifilm (1 mentions)
Bas 5000 phosphoimager, by Fujifilm (1 mentions)
3h microscales, by GE Healthcare (1 mentions)
Las 1000 image analyzer, by Fujifilm (1 mentions)
Nitrocellulose membrane, by Thermo Fisher Scientific (1 mentions)
Bis tris gel, by Thermo Fisher Scientific (1 mentions)
Sc 47724, by Santa Cruz Biotechnology (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
Tnt quick coupled transcription translation system, by Promega (1 mentions)
Fuji fla 3000 phosphor imager, by Fujifilm (1 mentions)
Anti v5, by Thermo Fisher Scientific (1 mentions)
Ecl advance western blotting detection kit, by GE Healthcare (1 mentions)
20 protocols using image gauge v4
1
Western Blot Image Analysis Protocol
2
Cytokine Profiling in Mouse Lung Models
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Four mice from each experimental group (PBS, BLM, and BLM-UNC0379) were used in a cytokine array. At 10 dpi of PBS or BLM, the trachea was exposed and lavaged three times with 1.2 ml ice-cold PBS using a 20-gauge catheter. After centrifuging the BAL fluid at 400 × g for 5 min, the resulting supernatants were stored at −80°C as samples for use in the cytokine array. The cell pellets were resuspended in PBS and stained with Diff-Quick (Sysmex Co., Kobe, Japan) for cell counting using a hemocytometer. For the cytokine array, the BAL fluids from two mice form each group were collected, mixed, and subjected to Proteome Profiler Mouse Cytokine Array Kit for 40 cytokines (ARY006; R&D systems, Inc., Minneapolis, MN). The WB cytokine array was performed twice according to the manufacturer’s instructions. The resulting signals for each cytokine were normalizing to the positive internal control included in the array membrane and evaluated using an Image Gauge V4.21 (FUJIFILM).
3
Quantitative Southern Blot Analysis
4
Ovary Lysate Preparation and Quantification
5
Quantifying HBV Encapsidated and Cytoplasmic pgRNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An RPA was performed as described previously [43 (link)] to analyze the encapsidated and cytoplasmic pgRNA. Radiolabeled anti-sense probes (446 nucleotides (nts); nts 1805–2187 of HBV sequence) were synthesized in vitro; the protected sequence comprised 369 nts. The relative levels of cytoplasmic pgRNA and pgRNA obtained from isolated core particles were measured using the Fujifilm Image Gauge V4.0 program.
6
Immunofluorescent Adipocyte Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The method for immunofluorescene study of mouse adipose tissue was followed as described [22] (link). Adipocyte area was measured from each section using Image Gauge v4.0 software (Fujifilm). Frequency, histograms and Gaussian distributions were calculated using Excel 2013 (Microsoft) and GraphPad Prism v6.0 (GraphPad Software).
7
Visualizing Phosphate Absorption in Arabidopsis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To visualize 33P absorption, 7-day-old Arabidopsis seedlings grown in high-phosphate medium were incubated for 1 day in liquid medium supplemented with 33P (400 Bq/mL) and exposed against an imaging plate (Fujifilm; Japan) for 4 days at -80°C. Radioluminographic images of the seedlings were then scanned using the FLA-5000 imaging analyzer (Fujifilm; Japan) and analyzed using Image Gauge v4.0 (Fujifilm; Japan).
8
Analyzing HBV DNA Synthesis by Southern Blotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To analyze HBV DNA synthesis by Southern blotting, HBV DNA extracted from isolated core particles was separated by agarose gel electrophoresis and hybridized to a 32P-labeled random-primed probe specific for the full-length HBV sequence, as described previously [43 (link)]. The relative intensities of HBV double-stranded linear (DL) DNA were measured using the Fujifilm Image Gauge V4.0 program (Fuji Film Science lab 2001).
9
Quantification of Gel Radioactivity and Protein
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Phosphorimages of dried radioactive gels were quantified using Image Gauge v. 4.0 (Fujifilm). DNA intensities from EtBr stained gels and the intensity of RNAP subunits was quantified via ImageLab v. 5.2 (BioRad). The number of replicates (n) for experiments is indicated in the Figure legends. Data are plotted as means ± standard deviations.
10
Quantifying PFKFB3 and Nrf2 Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were lysed using RIPA buffer for 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3) detection. To analyze nuclear factor erythroid 2-like 2 (Nrf2) levels, cytosolic and nuclear compartments were extracted first with cytosol extraction buffer containing HEPES (10 mM) pH 7.9, KCl (10 mM), EDTA (0.1 mM), 0.3% NP-40, and protease inhibitors and then with nucleus extraction buffer containing HEPES (20 mM) pH 7.9, NaCl (0.4 mM), EDTA (1 mM), 0.25% glycerol, and protease inhibitors. The cell lysate was subjected to Western blot analysis using anti-PFKFB3 antibody (Ab) (Abgent, San Diego, CA, USA), anti-Nrf2 Ab (Santa Cruz Biotechnology, Dallas, TX, USA), anti-actin Ab (Bethyl Laboratories Inc., Montgomery, TX, USA), or anti-lamin A/C Ab (Santa Cruz) as a primary Ab and horseradish peroxidase-conjugated anti-rabbit IgG Ab (Thermo Fisher Scientific) as a secondary Ab. Signals were detected using enhanced chemical luminescence (Amersham, Piscataway, NJ, USA). The immunoblot was digitized using an office scanner (UMAX Astra 4100, Taipei, Taiwan), and the intensity of the band of the expected molecular size was quantitated using Image Gauge V 4.0 (Fujifilm Corporation, Tokyo, Japan). The band intensity of PFKFB3 and cytosolic Nrf2 was normalized to actin, and nuclear Nrf2 was normalized to lamin.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!