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Mab2018

Manufactured by Abcam
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MAB2018 is a monoclonal antibody that recognizes the human CD73 (5'-Nucleotidase) protein. CD73 is an enzyme involved in the hydrolysis of extracellular adenosine monophosphate (AMP) to adenosine.

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Lab products found in correlation

Map2 antibody, by Abcam (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions) Ab209665, by Abcam (1 mentions) Af1700, by R&D Systems (1 mentions) Af1924, by R&D Systems (1 mentions) Sc 5279, by Santa Cruz Biotechnology (1 mentions) Af1979, by R&D Systems (1 mentions) Sc 8628, by Abcam (1 mentions) Axin2, by Abcam (1 mentions) Phospho rb s780, by Abcam (1 mentions) Foxg1, by Abcam (1 mentions) C myc, by Abcam (1 mentions)

3 protocols using mab2018

1

Immunocytochemical Analysis of Cell Cultures

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Cells were cultured on poly-D-lysine coated coverslips, fixed with 4% paraformaldehyde/PBS for 10 min and permeabilised with 0.2% Triton X100/PBS for 20 min. Blocking was carried out with 10% BSA in 0.1% Triton X100/PBS for 30 min. Primary antibodies (MYC antibody (9E10), Sox2 antibody (R&D, MAB2018), MAP-2 antibody (Abcam)) and the fluorescent-conjugated secondary antibodies were incubated at room temperature for 1 h. Staining was observed after mounting in mounting medium for fluorescence with DAPI (Vector). Error bars in Figures 
1C based on counting 15 cells and
3A, and
4A counting 100 cells.
Liu Y.R., Laghari Z.A., Novoa C.A., Hughes J., Webster J.R., Goodwin P.E., Wheatley S.P, & Scotting P.J. (2014). Sox2 acts as a transcriptional repressor in neural stem cells. BMC Neuroscience, 15, 95.
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2

Pluripotency Marker Expression Profiling

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SOX2 (1:100; R&D systems, MAB2018), TFAP2C (1:500, Abcam, ab218107 or 1:500, Santa Cruz, Sc12762 or 1:100, R&D systems, AF5059), OCT4 (1:200; Santa Cruz, sc5279 or 1:300, Santa Cruz, sc8628), TBXT (1:500; Abcam, ab209665), GATA2 (1:500; Abcam, ab173817), NANOG (1:400, Cell Signalling, 4893), KRT7 (1:100, Thermo Fisher, MA5-11986), GATA6 (1:100, R&D systems, AF1700), SOX17 (1:100, R&D systems, AF1924), OTX2 (1:300, R&D systems, AF1979).
Bergmann S., Penfold C.A., Slatery E., Siriwardena D., Drummer C., Clark S., Strawbridge S.E., Kishimoto K., Vickers A., Tewary M., Kohler T.N., Hollfelder F., Reik W., Sasaki E., Behr R, & Boroviak T.E. (2022). Spatial profiling of early primate gastrulation in utero. Nature, 609(7925), 136-143.
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3

Immunoblotting for Protein Analysis

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Immunoblotting was performed using standard protocols. Antibodies were diluted in 5% milk powder inTBS Tween 20 0.1%, and protein detection was carried out with HRP-coupled secondary antibodies and X-ray films. The following primary antibodies were used Axin2 (1:1000, Abcam 109307), SOX2 (1:400, R&D MAB2018), Phospho-Rb (S780) (1:500, Abcam 47763), c-MYC (1:1000, Abcam 32072), FOXG1 (1:50, hybridoma clone 17B12, Pollard lab), WIF1 (1:500, Abcam 186845), NF1 (1:500; Santa Cruz sc-67), PTEN (1:1000; CST 9556), phospho-EGFR Tyr1068 (1:1000; CST 3777), GAPDH (1:40000; ThermoFisher, 6C5), Actin (1:1000; Santa Cruz sc-1616). Quantification of band signal and normalisation to GAPDH signal was performed using ImageJ software and Excel.
Robertson F.L., O'Duibhir E., Gangoso E., Bressan R.B., Bulstrode H., Marqués-Torrejón M.Á., Ferguson K.M., Blin C., Grant V., Alfazema N., Morrison G.M, & Pollard S.M. (2023). Elevated FOXG1 in glioblastoma stem cells cooperates with Wnt/β-catenin to induce exit from quiescence. Cell reports, 42(6).
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