Used balloons were collected into vials for resveratrol analysis. Ethanol was added. The vials were firmly closed and intensely shaken in a test tube shaker for at least 30 s.
Treated coronary artery segments were dissected, placed in pre-weighted vials, and kept frozen until drug content analysis. For extraction, ethanol was added to the dissected segments to achieve an ethanol concentration of >50%. Samples were homogenized (Precelly 24 Dual Homogenizer, PEQLAB Biotechnologie GmbH, Erlangen, Germany).
All samples were extracted by ultrasonication at room temperature for 30 min followed by centrifugation at 17,500× g for 10 min.
Resveratrol extracted from balloon catheters and tissue samples was determined by HPLC with UV detection. Column: C18, 5 μm, 25 cm × 4.6 mm. Mobile phase: 45% phosphate buffer 0.005 M (pH 3.5) and 55% acetonitrile, 1 mL/min. Detection: 230 nm.
Retention time: 3.97 ± 0.02 min. Detection limit: <1 µg/mL. A standard solution was injected during the same run (resveratrol concentration 10 µg/mL).
Treated coronary artery segments were dissected, placed in pre-weighted vials, and kept frozen until drug content analysis. For extraction, ethanol was added to the dissected segments to achieve an ethanol concentration of >50%. Samples were homogenized (Precelly 24 Dual Homogenizer, PEQLAB Biotechnologie GmbH, Erlangen, Germany).
All samples were extracted by ultrasonication at room temperature for 30 min followed by centrifugation at 17,500× g for 10 min.
Resveratrol extracted from balloon catheters and tissue samples was determined by HPLC with UV detection. Column: C18, 5 μm, 25 cm × 4.6 mm. Mobile phase: 45% phosphate buffer 0.005 M (pH 3.5) and 55% acetonitrile, 1 mL/min. Detection: 230 nm.
Retention time: 3.97 ± 0.02 min. Detection limit: <1 µg/mL. A standard solution was injected during the same run (resveratrol concentration 10 µg/mL).