Blood samples were collected directly from the inferior vena cava using a 1-mL syringe at the end of the experiment. Serum was obtained by centrifugation at 2,000 × g for 10 min and stored at -70°C until use. Serum luteinizing hormone (LH), follicle stimulating hormone (FSH), and anti-Müllerian hormone (AMH) levels were measured using ELISA kits (Cusabio Biotech, Wuhan, China). Serum testosterone levels were measured using a Testosterone ELISA Kit (Abcam, Cambridge, United Kingdom). The 17β-estradiol (E2) levels were measured using an Estradiol (Rat) ELISA Kit (BioVision, United States). All hormone levels were measured according to the manufacturer’s instructions.
Estradiol rat elisa kit
Manufactured by Abcam
Sourced in United States
The Estradiol (Rat) ELISA Kit is a quantitative in vitro diagnostic assay used for the measurement of estradiol levels in rat serum or plasma samples. The kit employs the competitive enzyme immunoassay technique.
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4 protocols using estradiol rat elisa kit
1
Serum Hormone Quantification in Rats
2
Measurement of Serum Hormones in Rats
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Blood samples were collected directly from the inferior vena cava using a 1-mL syringe at the end of the experiment. Serum was obtained by centrifugation at 2000× g for 10 min and stored at −70 °C until use. Serum LH levels were measured using a rat LH ELISA kit (Cusabio Biotech, Wuhan, China). Serum testosterone levels were measured using a testosterone ELISA kit (Abcam, Cambridge, UK). 17β-Estradiol (E2) levels were measured using an estradiol (rat) ELISA kit (BioVision, Mountain View, CA, USA). All kits were used according to the manufacturers’ instructions.
3
Estrogen Level Changes After Surgery
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To determine the changes in estrogen levels after surgery, the estrogen levels in the rat serum were determined using an estradiol (rat) ELISA kit (Biovision Inc., Milpitas, CA, USA). The protein concentration was quantified by BCA before ELISA, and the same amount of protein from different culture conditions was loaded. Absorbance was measured at a wavelength of 450 and 550 nm. The 450 nm values were subtracted from the 550nm values for correction of the optical errors.
4
Hormonal Profiling via ELISA
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Serum luteinizing hormone (LH), follicle-stimulating hormone (FSH), was measured using enzyme linked immunosorbent assay (ELISA) kits (Sincere Biotech Co., Ltd, China). Serum testosterone (TET) levels were measured using a testosterone ELISA Kit (Ela science, United States). The 17β-estradiol (EST) levels were measured using an Estradiol (Rat) ELISA Kit (BioVision, United States). All hormone levels were measured according to the supplier’s instructions.
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