Log In Sign up
The largest database of trusted experimental protocols

CXCR7 is a lab equipment product that functions as a chemokine receptor. It is a member of the G protein-coupled receptor family and plays a role in cellular signaling processes.

Automatically generated - may contain errors

Lab products found in correlation

Cxcr4, by Thermo Fisher Scientific (3 mentions) Liquid dab substrate kit, by Roche (2 mentions) Biotinylated anti mouse secondary antibody, by Vector Laboratories (1 mentions) Dharmafect 1 reagent, by Thermo Fisher Scientific (1 mentions) Cxcr4, by Merck Group (1 mentions) Lipofectamine ltx plus reagent, by Thermo Fisher Scientific (1 mentions) Lentiviral shrna, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Hek293 cell, by American Type Culture Collection (1 mentions)

3 protocols using cxcr7

1

Immunohistochemical Analysis of CXCR4 and CXCR7

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry used standard procedures. Briefly, tumors were fixed in 4% paraformaldehyde and embedded in paraffin. Sections (4 μm) were deparaffinized and heated for 10 minutes in 10 mmol/L citrate buffer, pH 6.2, for antigen retrieval. They were stained with Harris solution and Eosin for histological examination and immunostained using the primary antibodies raised against CXCR4 (1:200; eBiosciences) and CXCR7 (1:75; ThermoScientific). Slides were then incubated for 30 min with secondary biotinylated anti-mouse antibody (dilution 1:200; Vector Laboratories Inc., Burlingame, CA). Immunostaining was developed with a liquid DAB substrate kit (Roche Diagnostics) according to the manufacturer’s instructions.
Romain B., Hachet-Haas M., Rohr S., Brigand C., Galzi J.L., Gaub M.P., Pencreach E, & Guenot D. (2014). Hypoxia differentially regulated CXCR4 and CXCR7 signaling in colon cancer. Molecular Cancer, 13, 58.
+ Open protocol
+ Expand
2

Quantifying Chemokine Receptor Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry used standard procedures with tumors fixed zinc fixative and embedded in paraffin. Tissue sections were incubated with the primary antibodies against CXCR4 (1:200; eBiosciences) and CXCR7 (1:75; ThermoScientific) after antigen retrieval and immunostaining was developed with a liquid DAB substrate kit (Roche).
Romain B., Benbrika-Nehmar R., Marisa L., Legrain M., Lobstein V., Oravecz A., Poidevin L., Bour C., Freund J.N., Duluc I., Guenot D, & Pencreach E. (2017). Histone hypoacetylation contributes to CXCL12 downregulation in colon cancer: impact on tumor growth and cell migration. Oncotarget, 8(24), 38351-38366.
+ Open protocol
+ Expand
3

Characterization of Rho GTPase Signaling in Breast Cancer and Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
MDA-MB-231 and HEK293 cells (ATCC) were maintained in DMEM (Gibco) supplemented with 10% FBS (v/v), 100 units/ml penicillin and 100mg/ml streptomycin at 37°C with 5% CO2.
RhoA, Net1, PDZ, LARG, p115, Ect2 (Santa Cruz); RhoC (Cell Signaling); CXCR4, NCKIPSD, tubulin, and CXCR7 (AbCam); CXCR4 (Sigma); and mDia2 (Proteintech) antibodies were used at 1:100–200 dilutions for immunoprecipitation (IP), western blotting, pull-down assays and immunofluorescence (IF).
MDA-MB-231s were transfected with Lipofectamine LTX/Plus reagent (Invitrogen) per manufacturer’s specifications. For siRNA transfections, Dharmafect ON-TARGETplus SMARTpools (Thermoscientific) against human NCKIPSD, DRF3, CXCR7, CXCR4 or GAPDH were used at 100nM with Dharmafect-1 reagent. Stable GEF knockdown cells were generated using lentiviral shRNA (Thermoscientific) (Supplemental Table 1) selected with 35µg/ml puromycin. Control cells were generated using empty pLKO1 or shGFPscr vector.
CFP-RhoA V14, CFP-RhoA V14I41A, CFP-RhoB V14, CFP-RhoB V14I41A, CFP-RhoC V14 and CFP-RhoC V14I41A were kind gifts from Dr. Art Alberts (Van Andel Institute, Grand Rapids, MI). Whole cell and IP lysates were prepared, and IPs were performed as described [8 (link)].
Wyse M.M., Goicoechea S., Garcia-Mata R., Nestor-Kalinoski A.L, & Eisenmann K.M. (2017). mDia2 and CXCL12/CXCR4 Chemokine Signaling Intersect to Drive Tumor Cell Amoeboid Morphological Transitions. Biochemical and biophysical research communications, 484(2), 255-261.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!