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Arg8 vasopressin

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[Arg8]-vasopressin is a synthetic peptide hormone that is structurally similar to the natural hormone vasopressin. It is commonly used in laboratory research as a pharmacological tool to study the physiological functions of vasopressin.

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Lab products found in correlation

Angiotensin 2, by Merck Group (2 mentions) Flipr calcium 5 assay kit, by Molecular Devices (2 mentions) Cell culture medium, by Thermo Fisher Scientific (2 mentions) Cell culture additives, by Thermo Fisher Scientific (2 mentions) Isoproternol, by Merck Group (2 mentions) Tc flash 2 in cell tetracysteine tag detection kit, by Thermo Fisher Scientific (2 mentions) Uk14303, by Merck Group (2 mentions) Anti phospho erk1 2 igg t202 y204 9101, by Cell Signaling Technology (2 mentions) Anti erk1 2 igg, by Cell Signaling Technology (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Horseradish peroxidase conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (2 mentions) Phenylephrine, by Merck Group (2 mentions) Sphingosine 1 phosphate (s1p), by Avanti Polar Lipids (2 mentions) Fugene hd transfection reagent, by Promega (2 mentions) Fura 2 am, by Thermo Fisher Scientific (1 mentions) Cell culture media, by Thermo Fisher Scientific (1 mentions) Fluo 4 am, by Thermo Fisher Scientific (1 mentions) Forskolin, by Merck Group (1 mentions) L glutamate, by Merck Group (1 mentions)

Spelling variants of this product

Vasopressin (16 citations) [Arg8]-Vasopressin acetate salt (6 citations)

5 protocols using arg8 vasopressin

1

G Protein-Coupled Receptor Signaling Assays

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Cell culture medium and cell culture additives were from Life Technologies (Grand Island, NY). FuGENE HD transfection reagent and Promega GloSensor™ cAMP reagent were from Fisher Scientific (Pittsburgh, PA). FLIPR Calcium 5 Assay Kit was from Molecular Devices, Inc. (Sunnyvale, CA). Lipofectamine 2000 and TC-FlAsH™ II In-Cell Tetracysteine Tag Detection Kits were from Invitrogen (Grand Island, NY). hPTH(1–34) was obtained from Bachem, Inc. (Torrance, CA). Angiotensin II, [Arg8]-vasopressin, isoproternol, phenylephrine, and UK14303 were from Sigma-Aldrich (St. Louis, MO). Sphingosine 1-phosphate (S1P) was from Avanti Polar Lipids Inc. (Alabaster, AL). SI was from MP Biomedicals (Santa Ana, CA). SVdF and SBpA were synthesized by the Department of Pharmacology and Therapeutics, McGill University (Montreal, Quebec, Canada). Rabbit polyclonal anti-arrestin2/3 was a gift from Robert J. Lefkowitz (Duke University, Durham, NC). Anti-phospho-ERK1/2 IgG (T202/Y204; #9101) and anti-ERK1/2 IgG (#4695) were from Cell Signaling Technology (Beverly, MA). Horseradish peroxidase-conjugated donkey anti-rabbit IgG was from Jackson Immuno-Research Laboratories, Inc. (West Grove, PA).
Lee M.H., Appleton K.M., Strungs E.G., Kwon J.Y., Morinelli T.A., Peterson Y.K., Laporte S.A, & Luttrell L.M. (2016). The conformational signature of arrestin3 predicts its trafficking and signaling functions. Nature, 531(7596), 665-668.
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2

Calcium Signaling in Parathyroid Cells

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Cell culture media, fura-2 AM, and fluo-4 AM were from Invitrogen. Human parathyroid hormone (PTH, residues 1-34) was from Bachem (Saffron Walden, UK). Other chemicals, including Arg8-vasopressin, were from Sigma-Aldrich.
Thurley K., Tovey S.C., Moenke G., Prince V.L., Meena A., Thomas A.P., Skupin A., Taylor C.W, & Falcke M. (2014). Reliable Encoding of Stimulus Intensities Within Random Sequences of Intracellular Ca2+ Spikes. Science signaling, 7(331), ra59.
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3

Vasopressin Signaling in Aquaporin Regulation

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Chemicals including [Arg8] vasopressin (AVP), Forskolin, 8-bromo-cAMP (8-Br-cAMP), dibutyryl-cAMP (db-cAMP) and H-89 were purchased from Sigma (St Louis, MO) and tolvaptan (V2 receptor antagonist) was obtained from Cayman. The AQP2 antibody was obtained from Sigma. Data are presented as means ±SD. We used paired Student's t-tests or one way ANOVA test to determine the significance of the difference between the control and experimental groups. Statistical significance was taken as P<0.05.
Fan L., Wang X., Zhang D., Duan X., Zhao C., Zu M., Meng X., Zhang C., Su X.T., Wang M.X., Wang W.H, & Gu R. (2015). Vasopressin-induced stimulation of the Na+-activated K+ channels is responsible for maintaining the basolateral K+ conductance of the thick ascending limb (TAL) in EAST/SeSAME syndrome. Biochimica et biophysica acta, 1852(11), 2554-2562.
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4

Vasopressin and Glutamate Protocol

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[Arg8]-vasopressin (1 mmol/L) and l-glutamate (500 mmol/L), both Sigma-Aldrich, were dissolved in water and the stock solutions kept in aliquots at −20°C.
Lykke K., Assentoft M., Fenton R.A., Rosenkilde M.M, & MacAulay N. (2015). Vasopressin receptors V1a and V2 are not osmosensors. Physiological Reports, 3(8), e12519.
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5

G Protein-Coupled Receptor Signaling Assays

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Cell culture medium and cell culture additives were from Life Technologies (Grand Island, NY). FuGENE HD transfection reagent and Promega GloSensor™ cAMP reagent were from Fisher Scientific (Pittsburgh, PA). FLIPR Calcium 5 Assay Kit was from Molecular Devices, Inc. (Sunnyvale, CA). Lipofectamine 2000 and TC-FlAsH™ II In-Cell Tetracysteine Tag Detection Kits were from Invitrogen (Grand Island, NY). hPTH(1–34) was obtained from Bachem, Inc. (Torrance, CA). Angiotensin II, [Arg8]-vasopressin, isoproternol, phenylephrine, and UK14303 were from Sigma-Aldrich (St. Louis, MO). Sphingosine 1-phosphate (S1P) was from Avanti Polar Lipids Inc. (Alabaster, AL). SI was from MP Biomedicals (Santa Ana, CA). SVdF and SBpA were synthesized by the Department of Pharmacology and Therapeutics, McGill University (Montreal, Quebec, Canada). Rabbit polyclonal anti-arrestin2/3 was a gift from Robert J. Lefkowitz (Duke University, Durham, NC). Anti-phospho-ERK1/2 IgG (T202/Y204; #9101) and anti-ERK1/2 IgG (#4695) were from Cell Signaling Technology (Beverly, MA). Horseradish peroxidase-conjugated donkey anti-rabbit IgG was from Jackson Immuno-Research Laboratories, Inc. (West Grove, PA).
Lee M.H., Appleton K.M., Strungs E.G., Kwon J.Y., Morinelli T.A., Peterson Y.K., Laporte S.A, & Luttrell L.M. (2016). The conformational signature of arrestin3 predicts its trafficking and signaling functions. Nature, 531(7596), 665-668.
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