Bms 754807

2-DG and mouse polyclonal Anti-actin antibody were purchased from Sigma-Aldrich (St. Louis, MO). BMS-754807 was obtained from Bristol-Myers Squibb through an MTA. Antibodies against AKT, phospho-AKT (p-AKTSer473), IGFR, phospho-IGFR (Tyr1135/1136), phospho-ACC, caspase-3, PARP, and MDR1 (cat#13978) were purchased from Cell Signaling Technology, Inc. (Beverly, MA). The MDR1 antibody recognizes both human and mouse MDR1. Mouse monoclonal anti-LKB1 antibody was purchased from Abcam. Anti-actin antibody was purchased from Sigma.

Growth media and supplements were purchased from Invitrogen (Grand Island, NY). IGF-I was purchased from GroPep (Adelaide, Australia). IGF-II was purchased from Gemini (Woodland, CA). Insulin was purchased from Eli Lilly (Indianapolis, IN). LY294002, U0126, and actin antibody were purchased from Sigma-Aldrich (St. Louis, MO). Humanized anti-IGF-IR monoclonal antibody huEM164 was provided by Immunogen Inc. (Norwood, MA). BMS-754807 was provided by Bristol Myers Squibb (New Jersey, Marco Gottardis). Antibodies for phosphorylated AKT serine 473, total and phospho-IGF-IR, total and phosphorylated p44/42 (MAPK), phospho-p38 ^MAPK, total and phospho-IRS-1 (serine 636/639), phospho-S6K1, p21, p18, CDK2, CDK6, cyclin D1, cyclin D3, and PARP were purchased from Cell Signaling Technology (Beverly, MA). The IRS-2, total ERα, IGF-IRα, InRβ antibodies, and protein agarose A were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Horseradish peroxidase-conjugated anti-phosphotyrosine (PY-20) was purchased from BD Biosciences (San Jose, CA). Anti-rabbit and anti-mouse horseradish peroxidase-conjugated secondary antibodies were purchased from Pierce (Rockford, IL). NT157 compound was kindly provided by A. Levitzki (The Hebrew University of Jerusalem, Israel).