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Plasmotest kit

Manufactured by Thermo Fisher Scientific
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Sourced in Germany

The PlasmoTest™ Kit is a diagnostic tool designed to detect the presence of Plasmodium parasites, the causative agent of malaria. The kit employs a rapid diagnostic test format to provide a qualitative result indicating the presence or absence of Plasmodium infection.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Sk n fi, by Leibniz Institute DSMZ (2 mentions) Imr 5 75, by Leibniz Institute DSMZ (2 mentions) Rpmi medium, by Thermo Fisher Scientific (1 mentions) 1640 media, by Thermo Fisher Scientific (1 mentions) Sk n be 2 cells, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

PlasmoTest Mycoplasma detection kits PlasmoTest

4 protocols using plasmotest kit

1

Establishing Stable NTRK1/2 Expression in SH-SY5Y Cells

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SH-SY5Y parental cells were maintained in RPMI Medium (Gibco) supplied with 10% fetal calf serum (FCS). Stable expression of NTRK1 or NTRK2 in SH-SY5Y human neuroblastoma cells was achieved as described before (27 (link)). SH-SY5Y-NTRK1 and SH-SY5Y-NTRK2 were cultivated in RPMI Medium, supplied with 10% FCS and 500 μg/ml G418 (Sigma). All cell lines underwent Short Tandem Repeat DNA genotyping for cell line identification as well as weekly testing for mycoplasma using the PlasmoTest™ Kit (Invitrogen). The general number of passages between thawing and use was <20 for all experiments performed.
Ali S., Toews K., Schwiebert S., Klaus A., Winkler A., Grunewald L., Oevermann L., Deubzer H.E., Tüns A., Jensen M.C., Henssen A.G., Eggert A., Schulte J.H., Schwich E., Rebmann V., Schramm A, & Künkele A. (2020). Tumor-Derived Extracellular Vesicles Impair CD171-Specific CD4+ CAR T Cell Efficacy. Frontiers in Immunology, 11, 531.
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2

Murine NXS2 Cell Culture Protocol

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The parental murine cell line NXS2 (16 (link)) was kindly provided by Holger N. Lode (HL) (University Medicine Greifswald, Germany). Cells were cultured in Dulbecco´s modified Eagle´s medium (DMEM, GIBCO, Darmstadt, Germany) supplemented with 10% fetal calf serum (FCS), 100 U/ml penicillin and 100 µg/ml streptomycin at 37°C in 5% CO2. Cultures were routinely tested for mycoplasma using the PlasmoTest™ Kit (Invitrogen GmbH, Darmstadt, Germany).
Ivasko S.M., Anders K., Grunewald L., Launspach M., Klaus A., Schwiebert S., Ruf P., Lindhofer H., Lode H.N., Andersch L., Schulte J.H., Eggert A., Hundsdoerfer P., Künkele A, & Zirngibl F. (2023). Combination of GD2-directed bispecific trifunctional antibody therapy with Pd-1 immune checkpoint blockade induces anti-neuroblastoma immunity in a syngeneic mouse model. Frontiers in Immunology, 13, 1023206.
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3

Neuroblastoma Cell Lines Culture Protocol

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The human neuroblastoma cells lines, NB69, SK-N-FI and IMR-5/75, were purchased from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (Braunschweig, Germany) and cultured in Roswell Park Memorial Institute (RPMI) 1640 media (GIBCO) supplemented with 10% fetal calf serum. SK-N-BE(2) cells were purchased from the American Type Culture Collection (Wesel, Germany), and cultured in Dulbecco’s modified Eagle’s medium (DMEM, GIBCO) supplemented with 10% fetal calf serum. The NXS2 murine neuroblastoma cell line19 (link) was kindly provided by Holger N. Lode (University Medicine Greifswald, Germany) and cultured in DMEM supplemented with 10% fetal calf serum, 100 U/mL penicillin and 100 µg/mL streptomycin. All cell lines were cultured at 37°C in 5% CO2. Cell line identity was assured by short tandem repeat DNA genotyping. Cultures were routinely tested for mycoplasma using the PlasmoTest Kit (Invitrogen).
Zirngibl F., Ivasko S.M., Grunewald L., Klaus A., Schwiebert S., Ruf P., Lindhofer H., Astrahantseff K., Andersch L., Schulte J.H., Lode H.N., Eggert A., Anders K., Hundsdoerfer P, & Künkele A. (2021). GD2-directed bispecific trifunctional antibody outperforms dinutuximab beta in a murine model for aggressive metastasized neuroblastoma. Journal for Immunotherapy of Cancer, 9(7), e002923.
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4

Cell Line Maintenance Protocol

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H1975, H358, H23, and H1703 cells were obtained from ATCC and maintained in RPMI-1640 media containing 10 % fetal bovine serum (FBS) and 1% Penicillin/Streptomycin. BEAS-2B and H460 cells were obtained from the Vanderbilt-Ingram Cancer Center Core Facility and maintained in the same conditions. 293T cells were obtained from ATCC and maintained in DMEM containing 10% FBS. All cells were cultured in a humidified incubator with 5% CO2 at 37 °C. Cell lines were used between passages 1 and 50 after thaw. Cell lines from ATCC were authenticated using short tandem repeat profiling. Mycoplasma testing was performed every 6 months, most recently in November 2019, using the PlasmoTest kit (Invitrogen).
Kim L.C., Rhee C.H, & Chen J. (2020). Rictor amplification promotes NSCLC cell proliferation through formation and activation of mTORC2 at the expense of mTORC1. Molecular cancer research : MCR, 18(11), 1675-1684.
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