Ho9810

Ovarian cancer cell lines, SKOV-3, HO9810, HO8910PM, and OVCAR3 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cell lines HO8910PM, HO9810, and OVCAR3 were cultured in RPMI-1640 culture medium, while SKOV-3 cells were cultured in McCoy’s 5A modified medium (Sigma-Aldrich, St. Louis MO, USA). All culture media were supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 μg/ml of streptomycin.

The ovarian cancer cell lines SK-OV-3, HO9810, HO8910PM, and OVCAR3 were bought from Cell Bank of the Chinese Academy of Sciences (Shanghai, People’s Republic of China). Ovarian cancer cell line OAW-42 was obtained from Sigma-Aldrich Co. (St Louis, MO, USA). SK-OV-3 and OAW-42 were cultured in McCOY’s 5A medium (Sigma-Aldrich Co.), while HO9810, HO8910PM, and OVCAR3 were cultured in RPMI-1640 medium, supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin, under 5% CO₂ in a humidified incubator at 37°C. The anti-HDGF(N-15) and anti-actin(I-19) antibodies were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). The HDGF enzyme-linked immunosorbent assay (ELISA) kit was obtained from Antibodies-online Company (Aachen, Germany).