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4 protocols using spex1403

1

Characterization of MoS2 and MoxW1-xS2 Alloy

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OM (Olympus DX51), Raman spectroscopy (HORIBA, Lab Ram ARAMIS; 532-nm laser excitation wavelength), AFM (VEECO, Multimode), PL (SPEX1403, SPEX; 532-nm laser excitation wavelength), absorbance with ultraviolet–visible spectrophotometer (JASCO Corporation, V-650), XPS (Thermo UK, K-alpha), SEM (JEOL Ltd, JSM-6701F), TEM (FEI Titan G2 Cube 60-300; accelerating voltage, 80 kV), STEM and EDX (JEM 2100F; accelerating voltage, 200 kV) analyses were employed to characterize the MoS2 and Mo1−xWxS2 alloy, and a VCC Mo1−xWxS2 multilayer.
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2

Comprehensive Characterization of SLS MoS2 Nanosheets

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OM (Nikon ECLIPSE LV100ND), SEM(JEOL-6701F), Raman spectroscopy (HORIBA, Lab Ram ARAMIS; 532 nm laser excitation wavelength), AFM (VEECO, Multimode), PL (SPEX1403, SPEX; 532 nm laser excitation wavelength), XPS (Thermo U.K., K-alpha radiation), TEM (FEI Titan G2 Cube 60-300, accelerating voltage = 80 kV) and a voltage/current meter (Keithley 4200, Keithley Instruments) were all used in characterizing the SLS MoS2 nanosheets.
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3

Fabrication of Troponin I Biosensor

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The conductive glass with ITO electrodes was sourced from Hua Nan Xiang Cheng Ltd. Troponin I and mouse anti-troponin I monoclonal antibodies were obtained from Shanghai Linc-Bio Science CO., LTD. 1-Pyrenebutyric acid N-hydroxysuccinimide ester (PBASE) was acquired from Shanghai Aladdin Biochemical Technology Co., Ltd. 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide-hydrochloride (EDC) and N-hydroxy succinimide (NHS) were purchased from Sigma-Aldrich. The troponin I aptamer sequence used, (5-NH2-CGT GCA GTA CGC CAA CCT TTC TCA TGC GCT GCC CCT CTT A-3′-6-FAM), was purchased from Sangon Biotech (Shanghai) Co., Ltd. Carboxylated ferric oxide nanoparticles were obtained from Nanjing XFNANO Materials Tech Co., Ltd. Dimethyl sulfoxide (DMSO) was purchased from Sangon Biotech (Shanghai) Co., Ltd. Sodium dodecylbenzene sulfonate (SDS) and sodium dodecyl sulfate phosphate-buffered saline (PBS, P5368-10PAK; pH 7.4) were obtained from Sigma-Aldrich (Shang-Hai, China). Bovine serum albumin (BSA) was purchased from Shanghai Linc-Bio Science CO., LTD. To evaluate the quality of graphene, a Raman microscopic system (SPEX-1403, SPEX) was utilized. High-magnification transmission electron micrographs (TEM) were captured using a JEOL JEM 2100F instrument.
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4

Characterization of BiVO4 Nanofibers and Ag2O-Doped Variants

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The morphologies, structures and crystalline forms of BiVO4 NFs and Ag2O-doped BiVO4 NFs were investigated by using a scanning electron microscope (SEM, MAGELLIAN-400), transmission electron microscope (TEM, JEM-2200FS), and X-ray powder diffractometer system (XRD, Rigaku D/max-2550), respectively. The change in the mass of a BiVO4 precursor as a function of temperature and time was assessed by acquiring thermogravimetric analysis and differential scanning calorimetry (TGA/DSC) curves in the isothermal mode using a TA SDT Q600 thermogravimetry analyzer. Raman spectra were acquired by using a Raman spectroscopy apparatus (Spex 1403) with a 514 nm-wavelength laser to identify the molecular structures of the samples. X-ray photoelectron spectroscopy (XPS) data were measured with an ESCALAB 250 electron spectrometer to characterize the surface chemical states of the samples. An absorption spectrum was obtained with a UV-Vis spectrophotometer (UV-2450), using BaSO4 as a reference.
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