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Hrp conjugate anti actin ac 15

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HRP conjugate anti actin (AC-15) is a laboratory reagent used for the detection and quantification of actin, a key structural protein, in biological samples. This product is a conjugate of horseradish peroxidase (HRP) and an antibody specific to the AC-15 epitope of actin. The HRP label allows for colorimetric or chemiluminescent detection of actin in various applications such as Western blotting, immunohistochemistry, and ELISA.

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Lab products found in correlation

Odyssey imaging system, by LI COR (2 mentions) Nitrocellulose membrane, by Bio-Rad (2 mentions) Anti chaf1a sc 10206, by Santa Cruz Biotechnology (2 mentions) Anti chaf1b sc 393662, by Santa Cruz Biotechnology (2 mentions) Anti tbp ab818, by Abcam (2 mentions) Anti ube2i 4786, by Cell Signaling Technology (2 mentions) Anti pcna d3h8p, by Cell Signaling Technology (2 mentions) Enhanced chemiluminescence, by PerkinElmer (2 mentions)

Spelling variants of this product

Anti-actin (1282 citations) AC-15 (141 citations) Actin-HRP (20 citations) Anti-actin AC-15 (17 citations) Anti-actin-HRP (12 citations)

2 protocols using hrp conjugate anti actin ac 15

1

Quantitative Western Blot Analysis

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Whole-cell lysates from reprogramming intermediates were run on 4-20% gradient SDS-polyacrylamide gels and transferred to nitrocellulose membrane (Bio-Rad) by standard methods. Membranes were blocked for 1 h in 5% non-fat dry milk in 1 × TBS with 0.05% Tween-20 (TBST), rinsed, and incubated with primary antibody diluted in 3% BSA in TBST overnight at 4°C. The following primary antibodies were used: anti-Chaf1a (sc-10206, Santa cruz), anti-Chaf1b (sc-393662, Santa Cruz), anti-TBP (ab818, Abcam), anti-Ube2i (4786, Cell signaling), anti-PCNA (D3H8P, Cell signaling), HRP conjugate anti actin (AC-15, Sigma). Blots were washed in TBST, incubated with HRP-conjugated secondary antibodies for semi-quantitative Western blot analysis and IRdye 800CW or IRdye 680RD for quantitative westerns, as indicated. Secondary antibodies for both methods were incubated in 5% milk in TBST for 1 hour at room temperature (except for anti-β-ACTIN-Peroxidase antibody, which was incubated for 15min), and washed again. HRP signal was detected by Enhanced ChemiLuminescence (Perkin Elmer). Fluorescent infrared signal was detected using LI-COR Odyssey imaging system.
Cheloufi S., Elling U., Hopfgartner B., Jung Y.L., Murn J., Ninova M., Hubmann M., Badeaux A.I., Ang C.E., Tenen D., Wesche D.J., Abazova N., Hogue M., Tasdemir N., Brumbaugh J., Rathert P., Jude J., Ferrari F., Blanco A., Fellner M., Wenzel D., Zinner M., Vidal S.E., Bell O., Stadtfeld M., Chang H.Y., Almouzni G., Lowe S.W., Rinn J., Wernig M., Aravin A., Shi Y., Park P., Penninger J.M., Zuber J, & Hochedlinger K. (2015). The histone chaperone CAF-1 safeguards somatic cell identity. Nature, 528(7581), 218-224.
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2

Quantitative Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole-cell lysates from reprogramming intermediates were run on 4-20% gradient SDS-polyacrylamide gels and transferred to nitrocellulose membrane (Bio-Rad) by standard methods. Membranes were blocked for 1 h in 5% non-fat dry milk in 1 × TBS with 0.05% Tween-20 (TBST), rinsed, and incubated with primary antibody diluted in 3% BSA in TBST overnight at 4°C. The following primary antibodies were used: anti-Chaf1a (sc-10206, Santa cruz), anti-Chaf1b (sc-393662, Santa Cruz), anti-TBP (ab818, Abcam), anti-Ube2i (4786, Cell signaling), anti-PCNA (D3H8P, Cell signaling), HRP conjugate anti actin (AC-15, Sigma). Blots were washed in TBST, incubated with HRP-conjugated secondary antibodies for semi-quantitative Western blot analysis and IRdye 800CW or IRdye 680RD for quantitative westerns, as indicated. Secondary antibodies for both methods were incubated in 5% milk in TBST for 1 hour at room temperature (except for anti-β-ACTIN-Peroxidase antibody, which was incubated for 15min), and washed again. HRP signal was detected by Enhanced ChemiLuminescence (Perkin Elmer). Fluorescent infrared signal was detected using LI-COR Odyssey imaging system.
Cheloufi S., Elling U., Hopfgartner B., Jung Y.L., Murn J., Ninova M., Hubmann M., Badeaux A.I., Ang C.E., Tenen D., Wesche D.J., Abazova N., Hogue M., Tasdemir N., Brumbaugh J., Rathert P., Jude J., Ferrari F., Blanco A., Fellner M., Wenzel D., Zinner M., Vidal S.E., Bell O., Stadtfeld M., Chang H.Y., Almouzni G., Lowe S.W., Rinn J., Wernig M., Aravin A., Shi Y., Park P., Penninger J.M., Zuber J, & Hochedlinger K. (2015). The histone chaperone CAF-1 safeguards somatic cell identity. Nature, 528(7581), 218-224.
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