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2 protocols using fumonisin b1 and b2

1

HPLC Analysis of Mycotoxin Contamination

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High-performance liquid chromatography (HPLC) grade methanol, acetonitrile, formic acid and ammonium formate (supplied by Sigma-Aldrich®, St. Louis, MO, USA). HPLC grade Milli Q® water was supplied by Biosciences East Central Africa (BecA), International Livestock Research Institute (ILRI), (P.O. Box 30709 Nairobi 00100, Kenya). Aflatoxin B1 was analysed by a multiple mycotoxin analysis method with mixed mycotoxins standards (Aflatoxin B1, B2, G1 and G2, Fumonisin B1 and B2, T-2 toxin, HT-2 toxin, and diacetoxyscirpenole) obtained from Sigma-Aldrich (Sigma-Aldrich Chemie B.V., Zwijndrecht, The Netherlands).
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2

Mycotoxin Analysis in Food Samples

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Acetonitrile, methanol, formic acid and 25% ammonium hydroxide (LC–MS grade) and ethyl acetate were obtained from Sigma-Aldrich, Schnelldorf, Germany. Evolute express ABN SPE columns were obtained from Biotage, Sweden. Water was purified on a Milli Q system (Millipore Corporation, USA). Aflatoxin B1, B2, G1, and G2, citrinin, cyclopiazonic acid, deoxynivalenol, enniatin A1 and B1, fumonisin B1 and B2, nivalenol, ochratoxin A, patulin, sterigmatocystin, T2 toxin and zearalenone were all obtained from Sigma-Aldrich.
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