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L arginine deficient silac dulbecco s modified eagle s medium dmem

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L-Arginine deficient SILAC Dulbecco's modified Eagle's medium (DMEM) is a cell culture medium specifically formulated without L-Arginine. It is designed for use in stable isotope labeling by amino acids in cell culture (SILAC) experiments.

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2 protocols using l arginine deficient silac dulbecco s modified eagle s medium dmem

1

Nano Drug Delivery System Development

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Hydrogenated soybean phosphatidylcholine (HSPC) and N-(carbonyl-methoxypolyethyleneglycol2000)-1,2-distearoyl-snglycero-3-phosphoethanolamine (DSPE-PEG) were purchased from NOF Corporation (Tokyo, Japan). Cholesterol was obtained from Tokyo Chemical Industry Co., Ltd (Tokyo, Japan). 1,1′-Dioctadecyltetramethyl indotricarbocyanine iodide (XenoLight DiR) was supplied by Caliper Life Sciences (Hopkinton, MA, USA). l-Arginine hydrochloride, doxorubicin, lipopolysaccharides (LPS) from Escherichia coli were purchased from Sigma Chemical Co. (St. Louis, MO, USA). l-Arginine deficient SILAC Dulbecco's modified Eagle's medium (DMEM) was obtained from Thermo Scientific (Waltham, MA, USA).
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2

Investigating Arginine-Doxorubicin Interactions

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L-Arginine hydrochloride, doxorubicin, N ω -nitro-L-arginine methyl ester hydrochloride (L-NAME), lipopolysaccharides (LPS) from Escherichia coli were all purchased from Sigma Chemical Co. (St. Louis, USA). Polycarbonate cell culture inserts in multi-dishes were obtained from Thermo Scientific™ Nunc TM (MA, USA). Trypsin-EDTA (0.25%) and L-arginine deficient SILAC Dulbecco's modified Eagle's medium (DMEM) were obtained from Thermo Scientific (MA, USA).
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