Betulinic acid was purchased from YuanYe biotechnology (Shanghai, USA) and dissolved in DMSO as 100 mM. Counting Kit-8 (CCK-8) assay and Annexin V-FITC Apoptosis kit were obtained from BestBio Company (Shanghai, China). mTOR antibody (ab2732), Caspase-3 antibody (ab2302), p62 antibody (ab155686) were provided by Abcam. After that, S6K1 antibody (CST 9202), p-S6K1 antibody (CST 9204S), AMPK antibody (CST 2532S), p-AMPKα1 antibody (CST 2537), p-mTOR antibody (CST 5536S), Caspase8 antibody (CST 4790), Bax antibody (CST 5023S) and LC3A/B antibody (CST 12741) were bought from Cell Signaling Technology. Bcl2 antibody (12789–1-AP) and GAPDH antibody (AP0063) were acquired from Proteintech and Bioworld Technology, respectively.
Bcl2 antibody 12789 1 ap
Manufactured by Proteintech
Bcl2 antibody (12789–1-AP) is a primary antibody developed by Proteintech. It is designed to detect the expression of the Bcl2 protein, which plays a key role in regulating apoptosis, or programmed cell death. The antibody is suitable for use in various immunoassay techniques, such as Western blotting, immunohistochemistry, and immunofluorescence.
Automatically generated - may contain errors
Lab products found in correlation
Bax antibody, by Cell Signaling Technology (1 mentions)
Caspase 8 antibody, by Cell Signaling Technology (1 mentions)
Ampk antibody, by Cell Signaling Technology (1 mentions)
S6k1 antibody, by Cell Signaling Technology (1 mentions)
P mtor antibody, by Cell Signaling Technology (1 mentions)
Betulinic acid, by Yuanye Bio-Technology (1 mentions)
Lc3a b antibody, by Cell Signaling Technology (1 mentions)
Annexin 5 fitc apoptosis kit, by BestBio (1 mentions)
2 protocols using bcl2 antibody 12789 1 ap
1
Betulinic Acid: Apoptosis and mTOR Pathway Modulation
2
PAGE Gel Analysis of Cell Signaling
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7.5% (PG111) and 12.5% (PG113) PAGE Gel Fast Preparation Kits were purchased from Epizyme Biotech Corp., Ltd (Shanghai, China). Gels were prepared according to the manufacturer’s instruction. Beta tubulin antibody (66240-1-Ig), BAX antibody (50599-2-Ig) and BCL2 antibody (12789-1-AP) were purchased from Proteintech (Wuhan, China). Anti-caspase-3 antibody (ab13847), anti-ATR (phospho T1989) antibody (ab227851), anti-Chk1 (phospho S345) antibody (ab47318) and anti-gamma H2A.X (phospho S139) antibody (ab11174) were purchased from Abcam (Shanghai) Corp., Ltd (China). Anti-Rac1 monoclonal antibody (ARC03) and anti-active Rac1-GTP mouse monoclonal antibody (26903) were purchased from Cytoskeleton (Denver, CO, USA) and NewEast Biosciences (King of Prussia, USA), respectively. HIF-1α rabbit monoclonal antibody (36169), PAK1 antibody (2602), phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody (2606), anti-rabbit IgG, HRP-linked antibody (7074) and anti-mouse IgG, HRP-linked antibody (7076) were purchased from Cell Signaling Technology (Shanghai) Corp., Ltd (China). Analysis of WB results was conducted by ImageJ software.
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