Hepes 1 m buffer solution

Manufactured by Thermo Fisher Scientific

Sourced in Sweden, United Kingdom

Hepes 1 M buffer solution is a commonly used buffer for maintaining a stable pH in biological and biochemical applications. It provides a pH range of 7.0 to 7.6 at 25°C. The solution is ready-to-use and does not require any additional preparation.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

HEPES buffer (1 M) HEPES buffer solution 1 M HEPES HEPES buffer HEPES solution MES buffer Buffer solutions HEPES

6 protocols using hepes 1 m buffer solution

Fluorofentanyl Metabolism in Human Hepatocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ortho-, meta- and para-fluorofentanyl were purchased from Cayman Chemicals (Ann Arbor, MI, USA). LC-MS grade acetonitrile, formic acid and methanol were purchased from Fisher Scientific (Gothenburg, Sweden). Ammonium formate was obtained from Sigma–Aldrich (Stockholm, Sweden) and 99.5% ethanol from Kemetyl (Haninge, Sweden). Divide; Cryo-preserved human hepatocytes (LiverPool™, 10-donor-pool, Lot nr. RBR) and InVitro Gro HT medium were from BioreclamationIVT (Baltimore, MD, USA). Williams medium E (without L-glutamine and phenol red), L-glutamine 200 mM and Hepes 1 M buffer solution from Gibco® by life technologies™ were purchased from Thermo (Stockholm, Sweden). MilliQ Gradient 10 production unit from Millipore (Billerica, MA, USA) was used to produce high-purity water. β-Glucuronidase/arylsulfatase stock solution (Helix promatia), with activities of 4.5 and 14 U/ml respectively, was purchased from Roche Diagnostics (Mannheim, Germany).

Gundersen P.O., Åstrand A., Gréen H., Josefsson M., Spigset O, & Vikingsson S. (2019). Metabolite Profiling of Ortho-, Meta- and Para-Fluorofentanyl by Hepatocytes and High-Resolution Mass Spectrometry. Journal of Analytical Toxicology, 44(2), 140-148.

+ Open protocol

+ Expand

Generation of Erlotinib-Resistant HCC827 Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cell line HCC827 was purchased from ATCC. An erlotinib-resistant HCC827 cell line (HCC827ER) was generated as previously described [21 (link)]. Further, HCC827ER clones were established using minimal dilution [21 (link)]. All cells were maintained in RPMI 1640 media supplemented with 1% Penicillin-Streptomycin (Gibco), 1% Hepes 1 M buffer solution (Gibco), 1% Sodium Pyruvate (Gibco), 10% Fetal Bovine Serum (Gibco), and 1% 250 μg/mL Amphotericin B solution (Sigma-Aldrich). Further, the resistant cell line and clones were maintained in 5 μM erlotinib (Selleckchem).
For all mRNA and flow cytometry experiments cells were plated in 6-well plates and incubated for 24 h (300,000 cells/well). After incubation the cells were treated with drug and incubated for 72 h prior to harvest.
The inhibitors erlotinib, crizotinib, and SCH772984 were all purchased from Selleckchem.

Demuth C., Andersen M.N., Jakobsen K.R., Madsen A.T, & Sørensen B.S. (2017). Increased PD-L1 expression in erlotinib-resistant NSCLC cells with MET gene amplification is reversed upon MET-TKI treatment. Oncotarget, 8(40), 68221-68229.

+ Open protocol

+ Expand

Culturing HEK-293 Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK-293T cells were from ATCC. HEK-293T-hACE2 cells were from YEASEN Biotech. HEK-293F cells were from Gibco. HEK-293T and HEK-293T-hACE2 cells were cultured in Dulbecco’s modified eagle medium (DMEM, Gibco), supplemented with 10% Fetal bovine serum (FBS, Gibco), 1% penicillin-streptomycin (Gibco), and 1% HEPES (1M) buffer solution (Gibco) at 37°C with 5% CO₂. HEK-293F cells were cultured in FreeStyle 293 expression medium (Gibco) at 37°C with 8% CO₂ at 130 rpm.

Ju B., Fan Q., Liu C., Shen S., Wang M., Guo H., Zhou B., Ge X, & Zhang Z. (2023). Omicron BQ.1.1 and XBB.1 unprecedentedly escape broadly neutralizing antibodies elicited by prototype vaccination. Cell Reports, 42(6), 112532.

+ Open protocol

+ Expand

Culturing HEK293 and Jurkat Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293F (Gibco) cells were cultured in suspension using FreeStyle 293 expression medium (Gibco) at 37°C with 8% CO₂ at 130 rpm. HEK293T (ATCC) and Jurkat-FcγRIIIa-V158 (Vazyme Biotech) cells were maintained in Dulbecco’s modified eagle medium (DMEM, Gibco) and Roswell Park Memorial Institute 1640 medium (RPMI-1640, Gibco), respectively, supplemented with 10% Fetal bovine serum (FBS, Gibco), 1% penicillin-streptomycin (Gibco) and 1% Hepes (1M) buffer solution (Gibco) at 37°C with 5% CO₂.

Wang M., Fan Q., Zhou B., Ye H., Shen S., Yu J., Cheng L., Ge X., Ju B, & Zhang Z. (2022). A key F27I substitution within HCDR1 facilitates the rapid maturation of P2C-1F11-like neutralizing antibodies in a SARS-CoV-2-infected donor. Cell Reports, 40(11), 111335.

+ Open protocol

+ Expand

Culturing Diverse DMG Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

All DMG cell lines used in the study (except for SF7761) were cultured in ultra-low attachment flasks in culture medium with 1:1 ratio of DMEM/F-12 (Invitrogen, 11330-032) and Neurobasal A (Invitrogen, 10888-022) and ten percent each of HEPES Buffer Solution 1 M (Thermo Fisher, 15630080), Sodium Pyruvate solution 100 nM (Life Technologies, 11360070), MEM non-essential amino acids solution 10 mM (Thermo Fisher, 11140050), Glutamax-I Supplement (Thermo Fisher, 35050061), and Penicillin/Streptomycin solution (Life Technologies, 15140122). The media was supplemented with B27 Minus Vitamin A (Invitrogen, 12587-010), epidermal growth factor (EGF; StemCell Tech. Inc., 78006), fibroblast growth factor (FGF; GF003, StemCell Tech., Inc., 78003) and heparin solution, 0.2% (StemCell Tech. Inc., 07980), as well as PDGF-AA (Shenandoah Biotech, 100-16) and PDGF-BB (Shenandoah Biotech, 100-18). SF7761 cell line was cultured in medium with Neurobasal A (Invitrogen, 10888-022) and N-2 Supplement (Invitrogen, 17502), further supplemented with B27 Minus Vitamin A (Invitrogen, 12587-010), epidermal growth factor (EGF; StemCell Tech. Inc., 78006), fibroblast growth factor (FGF; GF003, StemCell Tech. Inc., 78003) and heparin solution, 0.2% (StemCell Tech. Inc., 07980). Cells were dissociated using Accutase (StemCell Tech. Inc., 07922) and passaged every 3 or 4 days.

Khadka P., Reitman Z.J., Lu S., Buchan G., Gionet G., Dubois F., Carvalho D.M., Shih J., Zhang S., Greenwald N.F., Zack T., Shapira O., Pelton K., Hartley R., Bear H., Georgis Y., Jarmale S., Melanson R., Bonanno K., Schoolcraft K., Miller P.G., Condurat A.L., Gonzalez E.M., Qian K., Morin E., Langhnoja J., Lupien L.E., Rendo V., Digiacomo J., Wang D., Zhou K., Kumbhani R., Guerra Garcia M.E., Sinai C.E., Becker S., Schneider R., Vogelzang J., Krug K., Goodale A., Abid T., Kalani Z., Piccioni F., Beroukhim R., Persky N.S., Root D.E., Carcaboso A.M., Ebert B.L., Fuller C., Babur O., Kieran M.W., Jones C., Keshishian H., Ligon K.L., Carr S.A., Phoenix T.N, & Bandopadhayay P. (2022). PPM1D mutations are oncogenic drivers of de novo diffuse midline glioma formation. Nature Communications, 13, 604.

+ Open protocol

+ Expand

Culturing Patient-Derived DIPG Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Patient-derived cultures HSJD-DIPG-007 and ICR-B193 were grown in stem cell media consisting of Dulbecco's Modified Eagles Medium: Nutrient Mixture F12 (DMEM/F12), Neurobasal-A Medium, HEPES Buffer Solution 1M, sodium pyruvate solution 100nM, nonessential amino acids solution 10mM, Glutamax-I Supplement and penicillin Streptomycin solution (all Thermo Fisher, Loughborough, UK). The media was supplemented with B-27 Supplement Minus Vitamin A, (Thermo Fisher), 20ng/ml Human-EGF, 20ng/ml Human-FGFbasic-154, 20ng/ml Human-PDGF-AA, 20ng/ml Human-PDGF-BB (all Shenandoah Biotech, Warwick, PA, USA) and 2µg/ml Heparin Solution (0.2%, Stem Cell Technologies, Cambridge, UK) to constitute the complete media. In brief, cells were incubated at 37°C, 5% CO2, 95% humidity and were refed at least twice weekly with complete media. Cell authenticity was verified using short tandem repeat (STR) DNA fingerprinting.

Kessler K., Mackay A., Grabovska Y., Molinari V., Burford A., Temelso S., Tari H., Yara-Romero E., Liu I., Yu L., Castel D., Choudhary J.S., Sottoriva A., Filbin M.G., Vinci M., & Jones C. (2021). Loss of the H4 lysine methyltransferase KMT5B drives tumorigenic phenotypes by depleting H3K27me3 at loci otherwise retained in H3K27M mutant DIPG cells.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!