DNA was isolated from cervical swabs or cell cultures using the QiaSymphony robotic system (Qiagen) and the QiaSymphony Virus/Bacteria kit (Qiagen) with an 800 µl extraction protocol according to manufacturer’s instructions. Extracted DNA was subjected to bisulfite treatment using the Epitect DNA Methylation Kit (Qiagen). In brief, 200–500 ng of DNA (depending on the DNA concentration of the sample) were converted according to the low concentrated samples‐protocol of the manufacturer. During bisulfite treatment unmethylated cytosines (C) are delaminated, which are then converted into uracil (U), and replaced with thymine (T) during PCR amplification; methylated C nucleotides are protected from conversion and remain unmodified.
Qiasymphony virus bacteria kit
Manufactured by Qiagen
Sourced in United States
The QiaSymphony Virus/Bacteria kit is a nucleic acid purification solution designed for automated extraction of viral and bacterial nucleic acids from various sample types. The kit is compatible with the QIAcube Connect and QIAsymphony instruments, providing a streamlined and standardized sample preparation workflow.
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Lab products found in correlation
2 protocols using qiasymphony virus bacteria kit
1
DNA Bisulfite Conversion for Methylation Analysis
2
Detection of Bordetella pertussis in Nasopharyngeal Samples
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A total of 107 nasopharyngeal wash specimens were used in the present study, including selected PCR-positive (by a SYBR Green PCR [15 (link)]) specimens since August 2009, and specimens submitted between September 2011 to October 2012 to the Microbiology and Virology laboratory of BC Children’s Hospital for B pertussis PCR. Testing was performed exclusively on retrospective, residual samples that were stored at −80°C. To maintain patient anonymity, each sample was coded and all patient identifiers were removed to ensure that personnel involved in the present study were unaware of any patient information. Ethics approval was not considered to be necessary because studies that involve the secondary use of anonymous human biological materials are exempted from review by the local Research Ethics Board of the University of British Columbia. DNA from 0.2 mL nasopharyngeal wash specimens was extracted using the QIAsymphony virus/bacteria kit in an automated DNA extraction platform QIAsymphony SP (Qiagen, USA).
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