Cells were analyzed by flow cytometry with FACSForettsa or LSR II (BD) and FACSDiva software (BD Biosciences) or FlowJo software (Tree Star). The following monoclonal antibodies were used: anti-B220 (RA3–6B2), anti- CD19 (1D3), anti- CD3ε (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53-6.7), anti-CD11b (M1/70), anti-CD34 (RAM34), anti-CD45.1 (A20), anti-CD45.2 (104), anti-CD48 (HM48-1), anti-CD117 (2B8), anti-Flt3 (A2F10.1), anti-Gr-1 (RB6-8C5), anti-Sca-1 (D7), anti-CD71 (C2), anti-CD44 (IM7), anti-CD62L (MEL-14) and anti-Ter119 (TER119); from BD Biosciences; anti-CD25 (PC61) and anti-CD150 (TC15- 12F12.2) from Biolegend; anti-CD16/32 (93), anti-Ly6D (49-H4), anti-CD11C (N418) anti-NK1.1 (PK136)anti-CD41 (MWReg30), anti-CD105 (MJ7/18) and anti-CD127 (A7R34) from eBioscience. Cells incubated with biotinylated monoclonal antibodies were incubated with fluorochrome-conjugated streptavidin–Peridinin Chlorophyllprotein–Cyanine 5.5 (551419; BD Biosciences) or Streptavidin-Allophycocyanin-Cy7 (554063; BD Biosciences). In all the FACS plots, indicated are the relative frequencies (%) of the gated fraction.
Anti ter119 ter119
Manufactured by BD
Anti-Ter119 (TER119) is a lab equipment product used for flow cytometry analysis. It is a monoclonal antibody that specifically binds to the Ter119 antigen, which is expressed on the surface of erythroid cells. The core function of this product is to serve as a tool for the identification and enumeration of erythroid cells in various sample types.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd150 tc15 12f12.2, by BioLegend (5 mentions)
Streptavidin allophycocyanin cy7, by BD (5 mentions)
Facsdiva software, by BD (4 mentions)
Anti cd16 32 93, by Thermo Fisher Scientific (3 mentions)
Attune nxt, by BD (2 mentions)
Anti cd19, by BD (2 mentions)
Facs fortessa, by BD (2 mentions)
Attune nxt, by Thermo Fisher Scientific (2 mentions)
Anti cd127 a7r34, by Thermo Fisher Scientific (1 mentions)
Anti cd25 pc61, by BioLegend (1 mentions)
Streptavidin peridinin chlorophyllprotein cyanine 5, by BD (1 mentions)
Streptavidin super bright 650, by BioLegend (1 mentions)
Lsrfortessa, by BD (1 mentions)
Facsaria, by BD (1 mentions)
5 protocols using anti ter119 ter119
1
Multiparametric Flow Cytometry Analysis
2
Multiparametric Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were analyzed by flow cytometry with Attune Nxt (Thermofisher) and FlowJo software (Tree Star). The following monoclonal antibodies were used: anti- CD34 (RAM34), anti-CD45.1 (A20), anti-CD45.2 (104), anti-CD48 (HM48-1), anti-CD117 (2B8), anti-Flt3 (A2F10.1), anti-Sca-1 (D7), anti-B220 (RA3-6B2), anti- CD19 (1D3), anti-CD3 (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53-6.7), anti-CD11b (M1/70), anti– Gr-1 (RB6-8C5), and anti-Ter119 (TER119; from BD Biosciences); anti-CD150 (TC15-12F12.2) from Biolegend; anti-CD16/32 (93) from eBioscience. Cells incubated with biotinylated monoclonal antibodies were incubated with fluorochrome-conjugated streptavidin–peridinin chlorophyll protein–cyanine 5.5 (551419; BD), streptavidin-allophycocyanin-Cy7 (554063; BD), streptavidin-super bright 650 (Biolegend). In all the FACS plots, indicated are the percentages (%) of the gated fraction.
3
Multiparametric Flow Cytometry Analysis
4
Multiparametric Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were analyzed by flow cytometry with FACS Fortessa, LSR II (BD), Attune NXT and FACSDiva software (BD Biosciences) or FlowJo software (Tree Star). The following monoclonal antibodies were used: anti- CD34 (RAM34), anti-CD45.1 (A20), anti-CD45.2 (104), anti-CD48 (HM48-1), anti-CD117 (2B8), anti-Flt3 (A2F10.1), anti-Sca-1 (D7), anti-B220 (RA3-6B2), anti- CD19 (1D3), anti-CD3 (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53–6.7), anti-CDllb (M1/70), anti– Gr-1 (RB6-8C5) and anti-Ter119 (TER119; from BD Biosciences); anti-CD150 (TC15-12F12.2) from Biolegend; anti-CD16/32 (93) from eBioscience; anti-Mpl (AMM2) from IBL. Cells incubated with biotinylated monoclonal antibodies were incubated with fluorochrome-conjugated streptavidin–peridinin chlorophyll protein–cyanine 5.5 (551419; BD) or streptavidin-allophycocyanin-Cy7 (554063; BD). In all the FACS plots, indicated are the percentages (%) of the gated fraction.
5
Multiparametric flow cytometry analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were analyzed by flow cytometry with either LSRFortessa or LSRII (both BD) and FACSDiva software (BD) or FlowJo software (Tree Star). Cell sorting was performed with a FACSAria (BD). The following monoclonal antibodies were used: anti-B220 (RA3-6B2), anti-CD19 (1D3), anti-CD3ε (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53-6.7), anti-CD11b (M1/70), anti-CD34 (RAM34), anti-CD45.1 (A20), anti-CD45.2 (104), anti-CD48 (HM48-1), anti-CD117 (2B8), anti-Flt3 (A2F10.1), anti–Gr-1 (RB6-8C5), anti–Sca-1 (D7), and anti-Ter119 (TER119; from BD); anti-CD150 (TC15-12F12.2; from BioLegend); and anti-CD16/32 (93) and anti-CD127 (A7R34; from eBioscience). Cells incubated with biotinylated monoclonal antibodies were incubated with fluorochrome-conjugated streptavidin–peridinin chlorophyll protein–cyanine 5.5 (551419; BD) or streptavidin-allophycocyanin-Cy7 (554063; BD). In all of the FACS plots, the relative frequencies (%) of the gated fraction are indicated.
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