Ct rmc4550

Manufactured by Chemietek

The CT-RMC4550 is a rotational viscometer designed for measuring the viscosity of liquids. It consists of a motor-driven spindle that rotates at a constant speed and is immersed in the sample. The torque required to maintain the rotation is measured and used to calculate the viscosity of the liquid.

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Lab products found in correlation

Bms 345541, by Abcam (1 mentions) Mmcsf, by BioLegend (1 mentions)

2 protocols using ct rmc4550

Sphere Formation Assay for BTICs

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For the sphere formation assay, freshly dissociated cells were plated at 10,000 cells per well in 100 μl of BTIC medium into a 96-well flat bottom plate. The resultant number of spheres above the 60-μm-diameter cutoff, a convenient parameter to describe growth characteristics, was monitored after 3 to 5 days by bright-filed photographing multiple fields per well with subsequent analyzes as previously described (8 (link), 43 (link), 58 (link)). For the blockade of signaling pathways, BTICs were treated with the following inhibitors: p38 MAP Kinase Inhibitor Skepinone-l (Millipore, 506174), IKK-α/β inhibitor BMS 345541 (Abcam, ab144822), or RMC-4550 (Chemietek, CT-RMC4550).

Mirzaei R., Gordon A., Zemp F.J., Kumar M., Sarkar S., Luchman H.A., Bellail A.C., Hao C., Mahoney D.J., Dunn J.F., Bose P, & Yong V.W. (2021). PD-1 independent of PD-L1 ligation promotes glioblastoma growth through the NFκB pathway. Science Advances, 7(45), eabh2148.

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Isolation and Characterization of Murine Bone Marrow-Derived Macrophages

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To isolate bone marrow cells, mice were sacrificed and femur and tibia were harvested and sterilized in 70% ethanol for 10 min, rinsed in PBS. Bone marrow cells were collected by flushing bone marrow cavity RPMI medium. Bone marrow cell mixture was then processed to a single cell suspension through a 100μM filter (Cirning) and lysed of red cells. 5x10⁶ bone marrow cells were plated in a 10-cm culture dish in complete RPMI containing 50ng/mL of mM-CSF (Cat: 576402, BioLegend). Medium was refreshed on day 3. On day 6-7, macrophages were harvested with PBS+1mM EDTA. Flow cytometry analysis revealed >90% purity of CD45⁺CD11b⁺F4/80⁺ cells. For co-culture studies, macrophages were plated at a 1:1 ratio with indicated tumor cell lines. For treatment with RMC-4550 (ChemieTek, CT-RMC4550), co-cultures were treated with 1 μM RMC-4550 or vehicle (DMSO).

Farrar M.A., Campbell J.D, & Schreiber R.D. (1992). Identification of a functionally important sequence in the C terminus of the interferon-gamma receptor.. Proceedings of the National Academy of Sciences of the United States of America, 89(24), 11706-11710.

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