Caspase 5

Manufactured by Santa Cruz Biotechnology

Caspase-5 is a protein that plays a crucial role in the process of apoptosis, or programmed cell death. It is a member of the caspase family of enzymes, which are responsible for the proteolytic cleavage of various cellular substrates during apoptosis. Caspase-5 is involved in the activation of other caspases and the subsequent execution of the cell death program.

Automatically generated - may contain errors

Lab products found in correlation

Ag 20b 0014 c100, by Adipogen (2 mentions) Ve cadherin, by Santa Cruz Biotechnology (2 mentions) Af 401 na, by Santa Cruz Biotechnology (2 mentions) Caspase 4, by Santa Cruz Biotechnology (2 mentions) Ag 20b 0042 c100, by Novus Biologicals (2 mentions) Lipofectamine 3000 transfection reagent, by Thermo Fisher Scientific (2 mentions) Cytotox 96 non radioactive cytotoxicity assay kit, by Promega (2 mentions) Protease inhibitor cocktail, by Merck Group (2 mentions) β actin, by Merck Group (2 mentions) Polyclonal anti sars cov 2 spike glycoprotein, by BEI Resources (2 mentions) Albumin, by Merck Group (2 mentions) O dianisidine dyhydrochloride, by Merck Group (2 mentions) Il 1β, by R&D Systems (1 mentions)

2 protocols using caspase 5

Characterizing NLRP3 Inflammasome Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

We purchased antibodies against NLRP3 (AdipoGen, AG-20B-0014-C100), Caspase-1 (AdipoGen, AG-20B-0042-C100), caspase-11 (Novus Biologicals, NB-120–10454), IL-1β (R&D Systems, AF-401-NA), caspase-4 (Santa Cruz Biotechnology Inc., sc-56056), caspase-5 (Santa Cruz Biotechnology Inc., sc-393346), CREB (Cell Signaling, 48H2), VE-cadherin (Santa Cruz Biotechnology, sc-6458), and β-actin (Sigma, A-5316). Polyclonal anti-SARS-CoV-2 spike glycoprotein was obtained from BEI Resources. IL-1R antagonist Anakinra (IL-1RA, #407616) was obtained from Calbiochem; Anakirna (C₇₅₉H₁₁₈₆N₂₀₈O₂₃₂S_10.) is a recombinant biopharmaceutical and slightly modified version of the human interleukin 1 receptor antagonist protein-IL-1RA. Albumin (catalog A7906), O-Dianisidine dyhydrochloride (catalog D3252), and protease inhibitor cocktail (catalog P8340) were from Millipore Sigma. The CytoTox 96® Non-Radioactive Cytotoxicity Assay kit (G1780) were purchased from Promega. Enhanced chemiluminescence (ECL) Western blotting Detection Reagents and nitrocellulose membranes (Hybond-ECL) were from Amersham Biosciences Corp. Lipofectamine® 3000 transfection reagents were from Invitrogen.

Xiong S., Zhang L., Qadir A.S., Richner J.M., Class J., Rehman J, & Malik A.B. (2021). Interleukin-1RA Mitigates SARS-CoV-2–Induced Inflammatory Lung Vascular Leakage and Mortality in Humanized K18-hACE-2 Mice. Arteriosclerosis, Thrombosis, and Vascular Biology, 41(11), 2773-2785.

+ Open protocol

+ Expand

NLRP3 and Caspase-1 Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

We purchased antibodies against NLRP3 (AdipoGen; AG-20B-0014-C100), caspase-1 (AdipoGen; AG-20B-0042-C100), caspase-11 (Novus Biologicals; NB-120-10454), IL-1β (R&D Systems; AF-401-NA), caspase-4 (Santa Cruz Biotechnology, Inc; sc-56056), caspase-5 (Santa Cruz Biotechnology, Inc; sc-393346), CREB (Cell Signaling; 48H2), VE-cadherin (Santa Cruz Biotechnology; sc-6458), and β-actin (Sigma; A-5316). Polyclonal anti–SARS-CoV-2 spike glycoprotein was obtained from BEI Resources. IL-1R antagonist anakinra (IL-1RA, #407616) was obtained from Calbiochem; anakinra (C₇₅₉H₁₁₈₆N₂₀₈O₂₃₂S_10.) is a recombinant biopharmaceutical and slightly modified version of the human IL-1RA. Albumin (catalog A7906), O-dianisidine dyhydrochloride (catalog D3252), and protease inhibitor cocktail (catalog P8340) were from Millipore Sigma. The CytoTox 96 Non-Radioactive Cytotoxicity Assay kit (G1780) was purchased from Promega. Enhanced chemiluminescence Western blotting detection reagents and nitrocellulose membranes (Hybond ECL) were from Amersham Biosciences Corp. Lipofectamine 3000 transfection reagents were from Invitrogen.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!