Mir 506 3p

Small interference RNA targeting DLX6-AS1 (si-DLX6-AS1: 5ʹ-AAUAAAGAACACUUACACUACUG-3ʹ) together with its negative control (si-NC: 5ʹ- UUCUCCGAACGUGUCACGUTT-3ʹ) were assembled by Ribobio (Guangzhou, China). miR-506-3p mimics (miR-506-3p; Product ID: miR10002878-1-5), miR-506-3p inhibitors (anti-miR-506-3p; Product ID: miR20002878-1-5) and respective controls, including miR-NC (Product ID: miR1N0000001-1-5) and anti-miR-NC (Product ID: miR2N0000001-1-5) were purchased from Ribobio. The sequences of DLX6-AS1 and STAT2 were amplified and cloned into the pcDNA3.1 overexpression vector, named as pcDNA-DLX6-AS1 and pcDNA-STAT2 (Sangon, Shanghai, China), and pcDNA empty vector was served as the negative control (pcDNA-NC). For stable DLX6-AS1 knockdown, a lentiviral vector containing short hairpin RNA targeting DLX6-AS1 (sh-DLX6-AS1: 5ʹ-GGTTCAGTATAGATTTCTA-3ʹ) and its negative control (sh-NC: 5ʹ- TTCTCCGAACGTGTCACGT-3ʹ) were synthesized by Research-Bio Co., Ltd (Shanghai, China). These oligonucleotides (20 nM) and plasmids (1 µg) were transfected into SK-N-SH and LAN-6 cells (5×10⁴ cells) using Lipofectamine 3000 Reagent (Invitrogen, Carlsbad, CA, USA).