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Envision system hrp dab anti rabbit detection kit

Manufactured by Agilent Technologies
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The Envision+ system-HRP/DAB anti rabbit detection kit is a product from Agilent Technologies designed for use in immunohistochemistry (IHC) applications. The kit contains the necessary reagents to perform a specific detection of rabbit primary antibodies in tissue samples.

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EnVision + System-HRP (DAB) detection kit EnVision+System-HRP (DAB) kit EnVision+ System-HRP (DAB) EnVision DAB Detection System EnVision + System HRP anti rabbit Envision + kit/HRP (DAB) Envision DAB detection kit EnVision-HRP detection system Envision rabbit detection system EnVision+ Rabbit/HRP kit

2 protocols using envision system hrp dab anti rabbit detection kit

1

Detecting T and B Cells in Kidney Sections

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Detection of CD3 positive T cells (Dako, 3 μg/ml) and B220 positive B cells (R&D systems, 0.83 μg/ml) in 4 µm paraffin embedded kidney sections were performed as described previously36 (link). Envision+ system-HRP/DAB anti rabbit detection kit (K4011, Dako) and Polink-2 Plus HRP Anti-Rat DAB detection Kit (D46-15, Golden Bridge International) were used to detect CD3 and B220 respectively.
Dorraji S.E., Hovd A.M., Kanapathippillai P., Bakland G., Eilertsen G.Ø., Figenschau S.L, & Fenton K.A. (2018). Mesenchymal stem cells and T cells in the formation of Tertiary Lymphoid Structures in Lupus Nephritis. Scientific Reports, 8, 7861.
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2

Kidney Tissue Immunohistochemistry Staining

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Hematoxylin and eosin (H&E) staining, Alcian blue/periodic acid–Schiff (AB/PAS) staining, and detection of CD3 positive T cells (Dako, 3 μg/ml), B220 positive B cells (R&D systems, 0.83 μg/ml), and F4/80 positive macrophages (Biolegend, 1 μg/ml) in 4 μm paraffin embedded kidney sections were performed as described previously.28 (link),35 (link) Envision+ system-HRP/DAB anti-rabbit detection kit (K4011, Dako) was used to detect CD3, and Polink-2 Plus HRP anti-rat DAB detection kit (D46-15, Golden Bridge International) was used to detect B220 and F4/80.
Dorraji E.S., Oteiza A., Kuttner S., Martin-Armas M., Kanapathippillai P., Garbarino S., Kalda G., Scussolini M., Piana M, & Fenton K.A. (2021). Positron emission tomography and single photon emission computed tomography imaging of tertiary lymphoid structures during the development of lupus nephritis. International Journal of Immunopathology and Pharmacology, 35, 20587384211033683.
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