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Cytokeratin mnf 116

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Cytokeratin MNF-116 is a monoclonal antibody that recognizes a broad spectrum of cytokeratins found in various epithelial cells. It is a laboratory reagent used for the identification and characterization of epithelial cells in tissue samples.

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Lab products found in correlation

Cytokeratin ae1 ae3, by Agilent Technologies (2 mentions) Cytokeratin cocktail, by Agilent Technologies (1 mentions) Cytokeratin cam 5, by BD (1 mentions) P53 do 7, by Agilent Technologies (1 mentions) Ep1933y, by Abcam (1 mentions) Vimentin v9, by Agilent Technologies (1 mentions) Qwin standard v3, by Leica (1 mentions) α smooth muscle actin, by Agilent Technologies (1 mentions) Vimentin v9, by Leica (1 mentions) Bcor c 10, by Santa Cruz Biotechnology (1 mentions) Hmb45, by Agilent Technologies (1 mentions) Cd45 2b11 pd7 26, by Agilent Technologies (1 mentions) Cd34 qbend 10, by Agilent Technologies (1 mentions) Desmin d33, by Agilent Technologies (1 mentions) Ki 67 mib 1, by Agilent Technologies (1 mentions)

3 protocols using cytokeratin mnf 116

1

Comprehensive Histological Evaluation of Tissues

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Trichrome and periodic acid Schiff (PAS) using standard histologic techniques. Tissue was also stained with antibodies to collagen III (BioGenex, San Ramon, CA; Monoclonal clone HWD1.1; at 1:80 titration), cytokeratin AE1/AE3 (Dako, Carpinteria, CA; prediluted), Cytokeratin CAM 5.2 (BD Biosciences, San Jose, CA; prediluted) cytokeratin cocktail (AE1/AE3;CAM5.2), cytokeratin MNF-116 (Dako; at 1/100), and CD34 (Dako; Monoclonal clone QBEND; at 1:320 titration). IHC was performed with automated stainers similar to methods previously published [1 (link), 26 ].
Farris A.B., Ellis C.L., Rogers T.E., Lawson D., Cohen C, & Rosen S. (2016). Renal Medullary and Cortical Correlates in Fibrosis, Epithelial Mass, Microvascularity, and Microanatomy Using Whole Slide Image Analysis Morphometry. PLoS ONE, 11(8), e0161019.
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2

Immunohistochemical Profiling of FFPE Tumor Tissue

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Tumour tissue and the agarose-embedded close-to-patient cancer cells were formalin fixed and paraffin embedded (FFPE) before 4 μm sections were cut for both H&E and immunohistochemistry (IHC) analysis. IHC was performed using standard techniques and in line with the manufacturer's instructions for the following primary antibodies: Cytokeratin (MNF116, DAKO), EpCam (Ber-EP4, DAKO), CD44 (DF1485, DAKO), p53 (DO-7, DAKO), Vimentin (V9, DAKO), TFF3 (Abcam), and ALDH1A1 (EP1933Y, Abcam) (see online supplementary method S6). Sections were viewed with a Leica DMLB Bright-field Microscope (Leica-microsystems, Milton Keynes, UK) and images acquired with Leica QWin Standard v3 software. The presence of any characteristically stained cells was considered positive with respect to negative controls, and confirmed by a second blinded individual.
Saunders J.H., Onion D., Collier P., Dorrington M.S., Argent R.H., Clarke P.A., Reece-Smith A.M., Parsons S.L, & Grabowska A.M. (2016). Individual patient oesophageal cancer 3D models for tailored treatment. Oncotarget, 8(15), 24224-24236.
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3

Comprehensive Immunohistochemistry Protocol

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For immunohistochemistry, 2 μm thick sections were cut and antigens retrieved in an epitope retrieval solution of pH 8 (RE7116; Novocastra, Newcastle, UK) at 68 °C for 17 h in a stirred water bath. The antibody clones, dilutions and sources are as follows: CD99 (12E7, 1:25; Dako, Ely, UK), vimentin (V9, 1:100, Novocastra), CD31 (JC70, 1:100, Dako), CD34 (QBend10, 1:50, Dako), cytokeratin AE1/AE3 (1:100, Dako), CD45 (2B11+PD7/26, Dako), cytokeratin MNF116 (1:50, Dako), desmin (D33, 1:100, Dako), α-smooth muscle actin (SMA) (1A4, 1:200, Dako), epithelial membrane antigen (EMA) (E29, 1:100, Dako), HMB-45 (1:200, Dako), S100 (NCL-L-S100p, 1:1000, Novocastra), WT1 (C-19, 1:500, Santa Cruz, Insight Biotechnology Limited, PO Box 520, Wembley, Middlesex,UK), TLE1 (M-101, 1:50, Santa Cruz), ERG (Erg-1/2/3 C-1; 1:50, Santa Cruz), INI1 (1:25, BD Transduction Laboratories (BD Biosciences), Becton Dickinson UK, Oxford, UK), BCOR (C-10, 1:50, Santa Cruz), ETV4 (16, 1:50, Santa Cruz) and Ki-67 (MIB1, 1:200, Dako).
Alholle A., Karanian M., Brini A.T., Morris M.R., Kannappan V., Niada S., Niblett A., Ranchère-Vince D., Pissaloux D., Delfour C., Gonzalez A.M., Antonescu C.R., Sumathi V., Tirode F, & Latif F. (2018). Genetic analyses of undifferentiated small round cell sarcoma identifies a novel sarcoma subtype with a recurrent CRTC1-SS18 gene fusion. The Journal of pathology, 245(2), 186-196.
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