δ9 thc

Manufactured by Lipomed

Sourced in Switzerland

Δ9-THC is a pure compound that can be used for analytical purposes. It serves as a reference standard for the identification and quantification of Δ9-THC in various samples.

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Lab products found in correlation

1290 infinity series uhplc system, by Agilent Technologies (1 mentions) Api 4000, by AB Sciex (1 mentions)

2 protocols using δ9 thc

Quantification of Cannabinoids by LC-MS/MS

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A total of eight tissue-free standards were prepared to obtain a calibration curve for Δ9-THC, OH-THC (Lipomed, Arlesheim, Switzerland), COOH-THC (Lipomed, Arlesheim, Switzerland) and CBD (Trigal Pharm, Vienna, Austria), with concentrations in the range of 1–500 ng/mL. Quantification of cannabinoids in tissue samples was performed by LC-MS/MS using a 1290 Infinity series UHPLC System (1290 Infinity series, Agilent, Germany) coupled to a triple quadrupole mass spectrometer (API 4000^®, Sciex, Framingham, MA, USA). LC separation was achieved on a Nucleodur C18 Isis reverse-phase column (50 × 2.0 mm, 1.8 μm; Macherey-Nagel, Germany). The mobile phase system was 0.05% acetic acid in water (eluent A) and acetonitrile (eluent B) at a flow rate of 0.6 mL/min and 50 °C. Detection was performed in the positive electrospray ionization (ESI) mode applying the following detection parameters: curtain gas, 30; CAD gas, 6; gas 1, 50; gas 2, 60; ion spray voltage, 4500 V; source temperature, 500 °C. The LC-MS/MS system was operated with the Software Analyst^® Version 1.7.1 (Sciex, Framingham, MA, USA). Transitions were recorded using the multiple reaction monitoring (MRM mode, Table 1).

Dumbraveanu C., Strommer K., Wonnemann M., Choconta J.L., Neumann A., Kress M., Kalpachidou T, & Kummer K.K. (2023). Pharmacokinetics of Orally Applied Cannabinoids and Medical Marijuana Extracts in Mouse Nervous Tissue and Plasma: Relevance for Pain Treatment. Pharmaceutics, 15(3), 853.

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Cannabinoid Reference Standards Preparation

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Cannabidiol pharmaceutical grade was purchased from Fagron (Nazareth, Belgium). Reference standard solution Δ9-THC in ethanol (EtOH) was purchased from Lipomed AG (Arlesheim, Switzerland). Reference standard solution Δ9-THC-A in acetonitrile (ACN) was purchased from Dr. Ehrenstorfer-LGC Standards GmbH (Wesel, Germany). CBD and Δ9-THC were stored at 4 °C whereas Δ9-THC-A was stored at -20 °C.

De Leersnijder C., Duchateau C., De Braekeleer K, & Deconinck E. (2022). Relative response factors and multiple regression models in liquid chromatography to quantify low-dosed components using alternative standards-proof of concept: total Δ9-THC content in cannabis flowers using CBD as reference. Analytical and bioanalytical chemistry, 414(22).

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