In vivo monoclonal antibody (mAb) anti-mouse PD-1 (CD279) (clone RMP1-14) was purchased from Bioxcell (Catalog #BE0146, West Lebanon, NH). The antibody was administered intraperitoneally (i.p.) either alone or in combination with λ phage vaccine.
Anti mouse pd 1 cd279 clone rmp1 14
Manufactured by BioXCell
Anti-mouse PD-1 (CD279) (clone RMP1-14) is a monoclonal antibody that binds to the mouse programmed cell death-1 (PD-1) receptor. PD-1 is an immune checkpoint protein that plays a role in regulating T-cell activation and function.
Automatically generated - may contain errors
3 protocols using anti mouse pd 1 cd279 clone rmp1 14
1
Anti-PD-1 mAb and λ Phage Vaccine
2
Murine Immune Checkpoint Blockade
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RX-5902 was provided by Rexahn, Inc. (Rockville, MD) [22 ]. The drug was prepared in sterile miliQ H2O for in vivo dosing. The anti-mouse CTLA-4 (CD152, clone 9D9) and anti-mouse PD-1 (CD279, clone RMP1–14) monoclonal antibodies were obtained from Bio X cell (West Lebanon, NH). Nivolumab (anti-human PD-1) was obtained from the University of Colorado Hospital Pharmacy.
3
Cell Line Maintenance for Melanoma
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UNC0642 and Bafilomycin A1 (Baf.A1) were purchased from Sigma Aldrich. Anti-mouse PD-1 (CD279) clone RMP1-14 and the rat IgG2a isotype control clone 2A3 were purchased from Bio X Cell. Human melanoma cell lines (Supplementary Table S1) were maintained as described previously (20) (21) (22) (23) in RPMI1640 media (Gibco) supplemented with 10% FBS (Sigma Aldrich), in a humidified atmosphere of 5% CO 2 at 37 C. Mycoplasma testing was routinely performed to all cell lines prior to experimentation.
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