Lymphocyte separation liquid

Manufactured by Solarbio

Sourced in China

Lymphocyte separation liquid is a reagent used in laboratory procedures to isolate and purify lymphocytes from whole blood samples. It aids in the separation and concentration of these cells for downstream analysis and applications.

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Lab products found in correlation

Flow cytometry, by BD (2 mentions) Cd4 cd25 regulatory t cells isolation kit, by Miltenyi Biotec (1 mentions) Cd4 cd25 regulatory t cell isolation kit, by Miltenyi Biotec (1 mentions)

3 protocols using lymphocyte separation liquid

Regulatory T Cell Isolation

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To obtain Tcons, the spleens were collected from C57/BL6 mice, then ground and isolated by lymphocyte separation liquid (Solarbio). Regulatory T cells were isolated from Tcons according to the procedure of the CD4⁺ CD25⁺ Regulatory T Cells Isolation Kit (Miltenyi Biotec, Germany), and the rest of the cells were considered as non‐Tregs. Their purity and the ratio of viability were assessed by flow cytometry (Becton Dickinson, San Jose, CA, USA).

Mo L., Zhang X., Shi X., Wei L., Zheng D., Li H., Gao J., Li J, & Hu Z. (2018). Norcantharidin enhances antitumor immunity of GM‐CSF prostate cancer cells vaccine by inducing apoptosis of regulatory T cells. Cancer Science, 109(7), 2109-2118.

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Regulatory T cell Isolation Protocol

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To obtained conventional T cells (Tcons), the spleens were collected, grinded and then isolated by lymphocyte separation liquid (Solarbio). CD4⁺CD25⁺ Tregs were isolated from Tcons by CD4⁺CD25⁺Regulatory T cell isolation kit according to the manufacturers' instructions (Miltenyi Biotec, Auburn, CA, USA). To observe the purity of Tregs, cells were stained with FITC-labeled CD4 antibody (Miltenyi Biotec), PE-labled anti-CD25 antibody (Miltenyi Biotec) for 30 min at 4-8℃ in the dark and then measured by flow cytometry (Becton Dickinson, CA).

Chen Q., Mo L., Cai X., Wei L., Xie Z., Li H., Li J, & Hu Z. (2018). ICOS signal facilitates Foxp3 transcription to favor suppressive function of regulatory T cells. International Journal of Medical Sciences, 15(7), 666-673.

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Immune Cell Isolation and Characterization

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DiR, DOX, and cell culture media were obtained from Meilun Biotech Co. Ltd. DSPE-PEG_2K were obtained from A.V.T. (Shanghai) Pharmaceutical Co., Ltd. Anti-CD62L (APC), anti-CD44 (PE), anti-CD86 (APC), anti-CD80 (PE), anti-CD11c (FITC), anti-CD3 (PerCP-Cy5.5), anti-CD4 (PE), anti-CD8 (FITC) were acquired from BioLegend, Inc (USA). The lymphocyte separation liquid was provided by Beijing Solarbio Science & Technology Co., Ltd, China. Other reagents used in this study were of the analytical grades.

Wang C., Yu H., Yang X., Zhang X., Wang Y., Gu T., Zhang S, & Luo C. (2022). Elaborately engineering of a dual-drug co-assembled nanomedicine for boosting immunogenic cell death and enhancing triple negative breast cancer treatment. Asian Journal of Pharmaceutical Sciences, 17(3), 412-424.

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