To measure the cytokine levels in the serum, we used a Human TNFα Quantikine ELISA kit, a Human IL‐6 Quantikine ELISA kit, and a Human IL‐1ra Quantikine ELISA kit (R&D Systems) and read the absorbance in a microplate reader at 450/570 nm. Cytokine concentrations were calculated by spiking recombinant protein into 0.1 mL aliquots of serum.
Human il 1ra quantikine elisa kit
Manufactured by R&D Systems
Sourced in United States
The Human IL-1ra Quantikine ELISA kit is a quantitative sandwich enzyme immunoassay designed to measure human interleukin-1 receptor antagonist (IL-1ra) levels in cell culture supernates, serum, and plasma.
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2 protocols using human il 1ra quantikine elisa kit
1
Cytokine Quantification in Serum
2
Quantification of Mouse IL-1β and IL-1RA
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The mouse IL-1βIL-1F2 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA) and the human IL-1RA Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA) were used according to the manufacturer’s instructions to detect serum mouse IL-1β and serum IL-1RA, respectively.
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