PCL scaffold was placed on the bottom of a 24-well plate. Five hundred microliters of collagen gel (Corning, #354236) was cast on the top of the scaffold, after which CM-Dil (Invitrogen, #C7000)-labeled HUVECs were seeded at a density of 1 × 105 cells per mL. After 5 days of culture, the constructs were fixed with a 4% paraformaldehyde solution (Thermo Fisher, #AAJ19943K2). The cells were imaged with a confocal microscope. Z-stack images were taken at a 20 µm thickness. HUVEC migration and lumen formation were quantified based on the constructed 3D images.
Collagen gel
Manufactured by Corning
Sourced in United States
Collagen gel is a laboratory product that is made from purified collagen extracted from animal sources. It is used as a three-dimensional cell culture matrix to support the growth and differentiation of cells in vitro. The gel provides a biomimetic environment that promotes cell attachment, proliferation, and organization, which can be useful for a variety of cell-based studies and applications.
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Lab products found in correlation
4 protocols using collagen gel
1
In Vitro Angiogenesis Assay
2
Collagen Gel Contraction Assay with WNT4
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Contraction of collagen gels was performed in 96-well plates as previously reported [18 (link)]. In brief, collagen gel (Corning, Albany, NY, USA), matrigel (Corning, Albany, NY, USA), FM, FBS, and suspended fibroblasts (with or without 400 ng/mL WNT4 pretreatment) were gently mixed. Then, 100 μl of the mixture was added to each well of a 96-well plate and allowed to solidify at 37 °C for 30 min before complete medium was added. For inhibitory experiments, both gels and media were incubated with 10 μM ICG-001.
3
Collagen Gel Contraction Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Contraction of collagen gels was performed in 96-well plates as previously reported [18] . Brie y, collagen gel (Corning, NY, USA), matrigel (Corning, NY, USA), FM, FBS, and suspended broblasts (with or without 400 ng/mL WNT4 pretreatment) were gently mixed. Then, 100 µl of the mixture was added to each well in a 96-well plate and allowed to gel at 37°C for 30 min before the complete medium was added. For the inhibitory experiments, both gels and media were incubated with 10 μM ICG-001.
4
Collagen Gel Contraction Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Contraction of collagen gels was performed in 96-well plates as previously reported [18] . In brief, collagen gel (Corning, Albany, NY, USA), matrigel (Corning, Albany, NY, USA), FM, FBS, and suspended broblasts (with or without 400 ng/mL WNT4 pretreatment) were gently mixed. Then, 100 µl of the mixture was added to each well of a 96-well plate and allowed to solidify at 37°C for 30 min before complete medium was added. For inhibitory experiments, both gels and media were incubated with 10 μM ICG-001.
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