Peripheral blood mononuclear cells (PBMCs) were obtained from 100 ml of total blood collected in EDTA from jugular vein at day 0 prior to infection and days 3, 7 and 15 post-BTV infection. The samples were purified using Ficoll cushion (GE Healthcare) purification method as described in (36 (link)). PBMCs were frozen as described in (36 (link)). When necessary, PBMCs were cultured in PBMC culture medium (RPMI supplemented with 10% FBS (Sigma), 4 mM L-glutamine, 10 mM HEPES, 1% 100X non-essential aminoacids, 1 mM sodium pyruvate, 100U/mL penicillin/100 μg/mL streptomycin and 50nM β-mercaptoethanol (all from Invitrogen)).
Pbmc culture medium
Manufactured by Merck Group
PBMC culture medium is a sterile, isotonic, buffered solution designed for the in vitro culture and maintenance of peripheral blood mononuclear cells (PBMCs). It provides the necessary nutrients and growth factors required to support the survival and proliferation of these cells.
Automatically generated - may contain errors
2 protocols using pbmc culture medium
1
Peripheral Blood Mononuclear Cell Isolation for BTV Study
2
Peripheral Blood Mononuclear Cell Isolation for BTV Study
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Peripheral blood mononuclear cells (PBMCs) were obtained from 100 ml of total blood collected in EDTA from jugular vein at day 0 prior to infection and days 3, 7 and 15 post-BTV infection. The samples were purified using Ficoll cushion (GE Healthcare) purification method as described in (36 (link)). PBMCs were frozen as described in (36 (link)). When necessary, PBMCs were cultured in PBMC culture medium (RPMI supplemented with 10% FBS (Sigma), 4 mM L-glutamine, 10 mM HEPES, 1% 100X non-essential aminoacids, 1 mM sodium pyruvate, 100U/mL penicillin/100 μg/mL streptomycin and 50nM β-mercaptoethanol (all from Invitrogen)).
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