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Rabbit anti phospho histone3 ph3 antibodies

Manufactured by Abcam
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Rabbit-anti-phospho-histone3 (pH3) antibodies are a type of primary antibody that specifically recognizes the phosphorylated form of histone H3 protein. These antibodies are commonly used in research applications to detect and analyze cell division and mitotic processes.

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Lab products found in correlation

Chicken anti gfp, by Thermo Fisher Scientific (2 mentions) Alexafluor488 conjugated anti chicken, by Thermo Fisher Scientific (1 mentions) Inverted a1r spectral, by Nikon (1 mentions) Alexa fluor 594 conjugated anti rabbit, by Thermo Fisher Scientific (1 mentions) Alexafluor488 conjugated anti chicken secondary antibody, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 conjugated anti rabbit secondary antibody, by Thermo Fisher Scientific (1 mentions) Inverted a1r spectral microscope, by Nikon (1 mentions)

Close spelling variants of this product

Rabbit anti-phospho-Histone3 Rabbit anti-Histone3 Rabbit anti-pH3 Anti-rabbit antibodies Rabbit antibodies Rabbit anti-

2 protocols using rabbit anti phospho histone3 ph3 antibodies

1

Fluorescent Embryo Imaging with IF Staining

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Transgenic fluorescent embryos were embedded in 1% agarose in a glass‐bottom dish. Immunofluorescence double staining was performed with chicken‐anti‐GFP (1:400; Life Technologies) and rabbit‐anti‐phospho‐histone3 (pH3) antibodies (1:250; Abcam). We used AlexaFluor488‐conjugated anti‐chicken (1:1,000; Life Technologies) and AlexaFluor594‐conjugated anti‐rabbit (1:1,000; Life Technologies) secondary antibodies to reveal primary antibodies. Confocal imaging was performed using a Nikon inverted A1r spectral.
Cacialli P., Mailhe M., Wagner I., Merkler D., Golub R, & Bertrand J.Y. (2022). Synergistic prostaglandin E synthesis by myeloid and endothelial cells promotes fetal hematopoietic stem cell expansion in vertebrates. The EMBO Journal, 41(19), e108536.
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2

Fluorescent Embryo Imaging Protocol

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Transgenic fluorescent embryos were embedded in 1% agarose in a glass-bottom dish. Immunofluorescence double staining was performed as described previously57 (link), with chicken anti-GFP (1:400; Life Technologies) and rabbit anti-phospho-histone 3 (pH3) antibodies (1:250; Abcam). AlexaFluor488-conjugated anti-chicken secondary antibody (1:1000; Life Technologies) and AlexaFluor594-conjugated anti-rabbit secondary antibody (1:1000; Life Technologies) were subsequently utilized to reveal primary antibodies. Confocal imaging was performed using a Nikon inverted A1r spectral microscope.
Cacialli P., Mahony C.B., Petzold T., Bordignon P., Rougemont A.L, & Bertrand J.Y. (2021). A connexin/ifi30 pathway bridges HSCs with their niche to dampen oxidative stress. Nature Communications, 12, 4484.
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