Bortezomib was purchased from ChemieTek (Indianapolis, IN). Thapsigargin, cycloheximide, and propidium iodide (PI) and N-acetyl-L-cysteine (NAC) were purchased from Sigma-Aldrich (St. Louis, MO). Antibodies were purchased from the following sources: phospho-eIF2α (#3398S), GCN2 (#3302), PERK (#3192) and lamin A/C (#4777) (Cell Signaling Technology, Beverly, MA), eIF2α, (#44-728G, Invitrogen/Life Technologies, Grand Island, NY), HRI (#sc-30143), ubiquitin (#sc-8017) and ATF4 (#sc-200) (Santa Cruz Biotechnology, Santa Cruz, CA), phospho-GCN2 (#ab75836, Abcam, Cambridge, MA), β-actin (#A5316, Sigma-Aldrich), and anti-mouse/anti-rabbit HRP-labeled secondary antibodies (#W4021/#W4011, Promega, Madison, WI).
Anti mouse anti rabbit hrp labeled secondary antibodies
Manufactured by Promega
Anti-mouse/anti-rabbit HRP-labeled secondary antibodies are laboratory reagents used to detect and quantify target proteins in various immunoassays. They bind to the primary antibodies that recognize the target proteins and are labeled with horseradish peroxidase (HRP) enzyme, enabling colorimetric or chemiluminescent detection.
Automatically generated - may contain errors
Lab products found in correlation
Ubiquitin, by Santa Cruz Biotechnology (2 mentions)
Eif2α, by Thermo Fisher Scientific (2 mentions)
Phospho eif2α, by Cell Signaling Technology (2 mentions)
Bortezomib, by Chemietek (2 mentions)
Phospho gcn2, by Abcam (2 mentions)
Lamin a c, by Cell Signaling Technology (2 mentions)
P erk, by Cell Signaling Technology (2 mentions)
Propidium iodide, by Merck Group (2 mentions)
Cycloheximide (chx), by Merck Group (2 mentions)
β actin, by Merck Group (2 mentions)
Thapsigargin, by Merck Group (2 mentions)
N acetyl l cysteine, by Merck Group (2 mentions)
2 protocols using anti mouse anti rabbit hrp labeled secondary antibodies
1
Cellular Stress Response Signaling
2
Cellular Stress Response Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Bortezomib was purchased from ChemieTek (Indianapolis, IN). Thapsigargin, cycloheximide, and propidium iodide (PI) and N-acetyl-L-cysteine (NAC) were purchased from Sigma-Aldrich (St. Louis, MO). Antibodies were purchased from the following sources: phospho-eIF2α (#3398S), GCN2 (#3302), PERK (#3192) and lamin A/C (#4777) (Cell Signaling Technology, Beverly, MA), eIF2α, (#44-728G, Invitrogen/Life Technologies, Grand Island, NY), HRI (#sc-30143), ubiquitin (#sc-8017) and ATF4 (#sc-200) (Santa Cruz Biotechnology, Santa Cruz, CA), phospho-GCN2 (#ab75836, Abcam, Cambridge, MA), β-actin (#A5316, Sigma-Aldrich), and anti-mouse/anti-rabbit HRP-labeled secondary antibodies (#W4021/#W4011, Promega, Madison, WI).
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