No 14695 1 ap

Manufactured by Proteintech

No.14695-1-AP is a lab equipment product manufactured by Proteintech. It is designed for scientific research purposes. The core function of this product is to facilitate specific laboratory procedures, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation.

Automatically generated - may contain errors

Lab products found in correlation

No 14395 1 ap, by Proteintech (2 mentions) No 21898 1 ap, by Proteintech (2 mentions) Gapdh, by Beyotime (2 mentions) Af0006, by Beyotime (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Sds polyacrylamide gel, by GenScript (1 mentions) Loading buffer, by Beyotime (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Molecular imaging software, by Kodak (1 mentions) β actin, by Cell Signaling Technology (1 mentions) α sma, by Proteintech (1 mentions) Tom20, by Proteintech (1 mentions) Tim23, by Proteintech (1 mentions) Tgf β, by Proteintech (1 mentions) Lc3 1 2, by Proteintech (1 mentions)

3 protocols using no 14695 1 ap

Cardiac Fibrosis Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rats were anesthetized with iso urane (3.5%) and sacri ced after receiving HSD for 8 weeks. The hearts were removed, immediately frozen in liquid nitrogen and stored at -70 ℃ until use. Heart tissues or cultured cells were sonicated in RIPA lysis buffer and homogenized. The debris was removed and the supernatant was obtained after centrifugation at 12,000 g for 10 min at 4°C. About 30-50 μg proteins was loaded for electrophoresis, and probed with primary antibodies against collagen I (1:1000; No.14695-1-AP; Proteintech Co., Wuhan, China), α-SMA (1:1000; No.14395-1-AP; Proteintech Co.), TGF-β (1:1000;
No.21898-1-AP; Proteintech Co.). GAPDH (1:1000, AF0006; Beyotime Biotechnology Co., Shanghai, China) was used as internal control. Images were analyzed using the Image-Pro Plus software.

Zhao K., Mao Y., Ye X., Ma J., Sun L., Li P., & Li Y. (2021). MicroRNA-210-5p Alleviates Cardiac Fibrosis via Targeting Transforming Growth Factor-beta Type I Receptor in Rats on High Salt Diet.

+ Open protocol

+ Expand

Western Blot Protein Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proteins were extracted from tissues or cells using RIPA lysis buffer (Beyotime). After centrifugation, the supernatant was mixed with 5X loading buffer (Beyotime) and boiled. The denatured proteins were separated by 4–12% or 4–20% SDS polyacrylamide gel (GenScript) and electrophoretically transferred to PVDF membranes (Millipore). The membranes were blocked with 5% nonfat dry milk for 1 h and probed with antibodies against TIPARP (1:100, Abcam ab170817 for Fig. 2; 1:500 ab84664 for the others), GAPDH (1:1000, Cell Signaling Technology #5174), β-actin (1:1000, Cell Signaling Technology #3700), collagen type I (1:1000, Proteintech No. 14695-1-AP), collagen type IV (1:1000, Proteintech No. 55131-1-AP), fibronectin (1:1000, Proteintech No. 15613-1-AP), and α-SMA (1:1000, Abcam ab7817). The membranes were then incubated with peroxidase-linked goat anti-mouse/rabbit IgG secondary antibody (1:3000, Yeasen). Signals were captured on a BioSpectrum imaging system (Ultra-Violet Products) or Kodak Molecular Imaging Software (Kodak).

Zhang Y., Song M., Bi Y., Lei Y., Sun X, & Chen Y. (2022). TIPARP is involved in the regulation of intraocular pressure. Communications Biology, 5, 1386.

+ Open protocol

+ Expand

Comprehensive Cardiac Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Heart tissues or cultured cells were sonicated in RIPA lysis buffer and homogenized. The debris was removed and the supernatant was obtained after centrifugation at 12,000 g for 10 min at 4°C. About 30–50 μg proteins was loaded for electrophoresis, and probed with primary antibodies against collagen I (1:1000; No.14695-1-AP; Proteintech Co., Wuhan, China), α-SMA (1:1000; No.14395-1-AP; Proteintech Co.), TGF-β (1:1000; No.21898-1-AP; Proteintech Co.), LC3 I/II (1:1000; No.14600-1-AP; Proteintech Co., Wuhan, China), TIM23 (1:1000; No.11123-1-AP; Proteintech Co., Wuhan, China), TOM20 (1:1000; No.66777-1-Ig; Proteintech Co., Wuhan, China), OPTN (1:1000; No.10837-1-AP; Proteintech Co., Wuhan, China), COX4 (1:1000; No.11242-1-AP; Proteintech Co., Wuhan, China). GAPDH (1:1000, AF0006; Beyotime Biotechnology Co., Shanghai, China) was used as internal control. Images were analyzed using the Image-Pro Plus software.

Li Y., Zhao K., Hu Y., Yang F., Li P, & Liu Y. (2024). MicroRNA-142-3p alleviated high salt-induced cardiac fibrosis via downregulating optineurin-mediated mitophagy. iScience, 27(5), 109764.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!