Erythromycin

Manufactured by Bio Basic

Sourced in Canada

Erythromycin is a macrolide antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, primarily targeting bacterial ribosomes.

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Lab products found in correlation

5 protocols using erythromycin

Listeria-based Ovalbumin Delivery Protocol

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Listeria monocytogenes strain 10403s expressing whole cytoplasmic OVA (Lm-OVA) was kindly provided by Shomyseh Sanjabi (UCSF). Lm-OVA stocks frozen at −80°C were grown overnight at 37°C in BHI broth supplemented with 5μg/ml Erythromycin (Bio Basic, Amherst, New York). Then, overnight cultures were sub-cultured by diluting into fresh BHI broth supplemented with 5μg/ml Erythromycin and grown for 4 hours. Bacteria CFU was then quantified by measuring optical density at 600 nm. For primary infections, bacterial culture was then diluted to 5×10⁴ CFU/100μl in sterile 1X PBS and 100μl was injected per mouse i.v. via the retroorbital vein. For rechallenge infections, bacteria were diluted to 1×10⁵ CFU/100μl if the host mice were naive or 1×10⁶ CFU/100μl if the host mice were previously infected.

Levine L.S., Hiam-Galvez K.J., Marquez D.M., Tenvooren I., Madden M.Z., Contreras D.C., Dahunsi D.O., Irish J.M., Oluwole O.O., Rathmell J.C, & Spitzer M.H. (2021). Single-cell analysis by mass cytometry reveals metabolic states of early activated CD8+ T cells during the primary immune response. Immunity, 54(4), 829-844.e5.

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Antimicrobial Agents and Materials

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Tetracycline (TCy) and chloramphenicol were purchased from EMD Millipore (Germany). Ampicillin, erythromycin, and kanamycin sulfate were purchased from Bio BasicInc. (USA). Vancomycin was purchased from Calbiochem (USA). Sulfamethoxazole, ciprofloxacin, trimethoprim, and hippuric acid were obtained from Sigma–Aldrich (Germany). Quinine was obtained from Alfa Aesar (USA). CaCl₂.2H₂O, ZnCl₂, KCl, NaCl, and NH₄Cl were purchased from Showa (Japan). Polyvinylpyrrolidone (PVP, molecular weight = 58,000) was obtained from Acros Organics (USA). All reagents were reagent grade and were used without further purification. Ultrapure water prepared using a Milli-Q water system (Simplicity, Millipore) was used to prepare aqueous solutions.

Sudewi S., Chabib L., Zulfajri M., Gedda G, & Huang G.G. (2023). Polyvinylpyrrolidone-passivated fluorescent iron oxide quantum dots for turn-off detection of tetracycline in biological fluids. Journal of Food and Drug Analysis, 31(1), 177-193.

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HPLC Analysis of Strictinin from Pu'er Tea

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All chemicals were purchased from E. Merck Co. (Merck KGaA, Darmstadt, Germany) unless stated otherwise. Erythromycin, neomycin, tetracycline, and amoxicillin were purchased from Bio Basic Inc. (Markham, Ontario, Canada); gentamicin, cefepime, cefmetazole, ceftazidime, and piperacillin were bought from Sigma-Aldrich; flomoxef was purchased from Shionogi (Osaka, Japan). High-performance liquid chromatography (HPLC)-grade acetonitrile and methanol were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Acetic acid (99.7%) was obtained from J.T. Baker (Mallinckrodt Baker, Inc., Phillipsburg, NJ, USA), and double-distilled water was supplied by a Millipore clear water purification system (Direct-Q; Millipore, Billerica, MA, USA). Pu’er tea prepared from the leaves of Yunnan wild trees (Camellia sinensis var. assamica) in 2008 was purchased from a local store in Taiwan, and used to purify strictinin.

Hsieh S.K., Xu J.R., Lin N.H., Li Y.C., Chen G.H., Kuo P.C., Chen W.Y, & Tzen J.T. (2016). Antibacterial and laxative activities of strictinin isolated from Pu’er tea (Camellia sinensis). Journal of Food and Drug Analysis, 24(4), 722-729.

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Listeria-based OVA Antigen Delivery

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Listeria monocytogenes strain 10403s expressing OVA (Lm-OVA) was kindly provided by Shomyseh Sanjabi (UCSF). Lm-OVA stocks frozen at -80 C were grown overnight at 37˚C in BHI broth supplemented with 5 ug/ml Erythromycin (Bio Basic, Amherst, New York). Then, overnight cultures were sub-cultured by diluting into fresh BHI broth supplemented with 5 ug/ml Erythromycin and grown for 4 hours. Bacteria CFU was then quantified by measuring optical density at 600 nm. Bacteria were then diluted to 5×10 4 CFU / 100µl in sterile PBS and 100 µl was injected per mouse i.v. via the retroorbital vein.

Levine L.P., Hiam K., Marquez D.M., Tenvooren I., Contreras D.C., Rathmell J.C., & Spitzer M.H. (2020). Single-cell metabolic dynamics of early activated CD8 T cells during the primary immune response to infection.

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Listeria and MCMV Infection Model

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Listeria monocytogenes strain 10403s expressing OVA (Lm-OVA) was kindly provided by Shomyseh Sanjabi (UCSF). Lm-OVA stocks frozen at -80 o C were grown overnight at 37˚ C in BHI broth supplemented with 5 ug/ml erythromycin (Bio Basic, Amherst, New York). Then, overnight cultures were sub-cultured by diluting into fresh BHI broth supplemented with 5 ug/ml erythromycin and grown for 4 hours. Bacteria CFU was then quantified by measuring optical density at 600 nm. Bacteria were then diluted to 5×10 4 CFU / 100µl in sterile PBS and 100 µl was injected per mouse i.v. via the retroorbital vein. MCMV Smith strain was kindly provided by Lewis Lanier (UCSF). MCMV was passaged in BALB/c mice and a salivary gland stock was prepared as previously described 28 . Mice were infected with MCMV by i.p. injection with 1 x 10 4 pfu.

Spitzer M.H., Hiam-Galvez K.J., DeBarge R., Lareau C.A., Cho N.W., Yee J.L., & Okholm T.L.H. (2021). Transient dendritic cell activation diversifies the T cell response to acute infection.

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