Staphylococci were identified to species level based on colony morphology, Gram staining, catalase production, coagulase testing (Slidex Staph Plus; bioMérieux, Marcy l’ Etoile, France) and by the Vitek2 System (GP card, bioMerieux). Susceptibility to cefoxitin (FOX), penicillin (PEN), erythromycin (ER), clindamycin (CLI), tobramycin (TOB), gentamicin (GM), ciprofloxacin (CIP), fusidic acid (FA), rifampicin (RIF), tetracycline (TET) and sulfamethoxazole/ trimethoprim (SXT), was tested by the disk diffusion method according to EUCAST guidelines [36 ]. Testing for inducible and constitutive lincosamide resistance was performed with the D-test. MICs of mupirocin (MUP) and oxacillin (OX) were determined by E-test® (bioMerieux). Isolates resistant to at least three classes of antimicrobials were considered multidrug resistant. Phenotypic determination of MSSA was based on cefoxitin disk susceptibility [36 ]. Isolates with mupirocin MIC > 1 mg/L were further analysed.
Vitek2 system gp card
Manufactured by bioMérieux
Sourced in France
The Vitek2 System is a compact and fully automated microbiology testing platform designed for the identification and antibiotic susceptibility testing of various microorganisms. The GP card is a specialized test card used with the Vitek2 System for the identification of Gram-positive bacteria.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using vitek2 system gp card
1
Staphylococcus Identification and Antimicrobial Susceptibility
2
Surveillance of Antibiotic Resistance in S. aureus
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A total of 1011 S. aureus isolates (1 isolate/patient) were collected from blood cultures in 16 Spanish hospitals during 2018-19 (12 hospitals, 12 months; and 4 hospitals, 6-9 months) (Table S1, available as Supplementary data at JAC Online). 15 Staphylococci were identified to species level based on colony morphology, Gram staining, catalase production, coagulase testing (i.e. Slidex Staph Plus; bioMe ´rieux, Marcy-l'E ´toile, France), MALDI-TOF MS, the Vitek2 System (GP card; bioMe ´rieux) or the MicroScan WalkAway System (Beckman Coulter), depending on each hospital's procedures. Resistance to amikacin, cefoxitin, chloramphenicol, ciprofloxacin, clindamycin, daptomycin, erythromycin, fosfomycin, fusidic acid, gentamicin, linezolid, mupirocin, penicillin, teicoplanin, tetracycline, tobramycin, trimethoprim/sulfamethoxazole and vancomycin was assessed using automatic methods (Table S1). Breakpoints were considered according to EUCAST and/or CLSI, depending on each hospital. MSSA-PEN S isolates of this collection (156 isolates) were included in the present multicentre study.
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