Total RNA was isolated from paired tumor and normal tissues using TRIzol reagent (Takara) following the manufacturer's instructions. RNA from the cytoplasm and nucleus of ESCC cells were separated using the PARIS™ Kit (Life Technologies). Quantitative real‐time PCR (qRT‐PCR) analysis was performed using the TB Green Premix Ex Taq (Takara, Japan) and the Biosystems 7900 Real Time PCR System (Thermo Fisher Scientific). Relative expression levels of genes were quantified using the 2−ΔΔCt method. The primer sequences are displayed in Table S1 .
Biosystems 7900 real time pcr system
Manufactured by Thermo Fisher Scientific
Sourced in Japan
The Biosystems 7900 Real Time PCR System is a laboratory instrument designed for quantitative real-time polymerase chain reaction (qPCR) analysis. It is capable of detecting and quantifying nucleic acid sequences in real-time during the amplification process.
Automatically generated - may contain errors
Lab products found in correlation
Paris kit, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Takara Bio (1 mentions)
Tb green premix ex taq, by Takara Bio (1 mentions)
Sybr green pcr master mix, by Roche (1 mentions)
Revertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
2 protocols using biosystems 7900 real time pcr system
1
Cytoplasmic and Nuclear RNA Isolation and Quantification
2
RNA Extraction and qRT-PCR Analysis
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Trizol reagent (Invitrogen, 15596018, USA) was used to isolated cell total RNA. Then, the RNA was used as a template strand for reverse transcription into cDNA using RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific,00887496) according to the operation manual. Finally, Biosystems 7900 Real Time PCR System (Thermo Scientific) and SYBR Green PCR Master Mix (Roche) were used to analyze the cDNA following the Instruction manual. GAPDH was used as an internal control. The 2-ΔΔCt method was used to calculate the relative quantitative value. All primers used are listed in Supplementary Table 2 .
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