Maximum intensity projections of 60 min time-lapses acquired every minute were analysed using a manual tracking plugin in Fiji. Measured data were transferred into a Chemotaxis and Migration tool programme (Ibidi) to design tracking and rose plots (Fig. 2 g,h for rose plots, Figs. 3 g,h and 4 g,h for tracking plots). A rose diagram maps single counts of the position of every macrophage in a selected area (black and grey sectors of angle π/18) every minute over 60 min with an (x,y 0,0) starting point. The tracking plot diagram represents the migration path and distance of macrophages in the AGM with an x,y 0,0 starting point, being measured every minute over 60 min. The average of single macrophage velocities (µm min−1) during 15–60 min were used for analysis. The evaluation of the directionality was performed using a Rayleigh statistical test for the uniformity of a circular distribution of points (end points of single macrophages). All analyses were conducted using the Chemotaxis and Migration tool software (Ibidi).
Chemotaxis and migration tool programme
Manufactured by Ibidi
The Chemotaxis and Migration tool programme is a software tool designed to facilitate the analysis of cell migration and chemotaxis. The core function of this programme is to provide a standardized and automated approach to quantifying and visualizing cell movement in response to various stimuli.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using chemotaxis and migration tool programme
1
Macrophage Migration Tracking and Analysis
2
Macrophage Migration Imaging Analysis
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Maximum intensity projections of 60 minute time-lapses acquired every minute were analysed using a manual tracking plugin in Fiji. Measured data were transferred into a Chemotaxis and Migration tool programme (Ibidi) to design tracking and rose plots (Figure 2G-H for rose plots, 3G-H and 4G-H for tracking plots). A rose diagram maps single counts of the position of every macrophage in a selected area (black and grey sectors of angle π/18) every minute over 60 minutes with an (x,y 0,0) starting point. The tracking plot diagram represents the migration path and distance of macrophages in the AGM with an x,y 0,0 starting point, being measured every minute over 60 minutes. The average of single macrophage velocities (µm min -1 ) during 15-60 minutes were used for analysis. The evaluation of the directionality was performed using a Rayleigh statistical test for the uniformity of a circular distribution of points (end points of single macrophages). All analyses were conducted using the Chemotaxis and Migration tool software (Ibidi).
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